EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY

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Abedi Gaballu Fereydoon; MANSOORI BEHZAD; DEHGHAN GHOLAMREZA

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Journal: Studies in Medical Sciences Year:2019 | Volume:30 | Issue:5 Page(s): 381-392

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Title

EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY

Author(s)

Abedi Gaballu Fereydoon | MANSOORI BEHZAD | DEHGHAN GHOLAMREZA | Issue Writer Certificate

Keywords

Methotrexate

dendrimer

apoptosis

cytotoxicity

cellular uptake

Abstract

Background & Aims: Poly (amidoamine) dendrimer (PAMAM) is highly macromolecular at nanosize with widely active amine groups on the surface that allows it to attach to the anti-cancer drugs such as Methotrexate (MTX). This study aimed to synthesize and characterize PAMAM-MTX (dendrimerMTX) complex, then to evaluate the cytotoxic effect of the synthesized complex on MCF-7 cancer cells. Materials & Methods: To perform this experimental study, initially, MTX was conjugated to the G4 dendrimer using Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), then it was characterized by Fourier-transform infrared (FTIR) and dynamic light scattering (DLS). Quantitative cellular uptake of nanoparticles was carried out using flow cytometry. The methylthiazol tetrazolium (MTT) and 4′ , 6Diamidino-2-phenylindole dihydrochloride (DAPI) staining assays were used for evaluating the ability of dendrimer-MTX and MTX in inducing cell cytotoxicity and apoptosis on MCF-7 cancer cells, respectively. Results: The results of this study illustrated that the dendrimer-MTX complex had optimal size (30 ± 7. 29 nm) and zeta potential (5. 35 ± 4. 37 mV). Flow cytometry analysis revealed that the cellular uptake was increased (18%) after conjugating MTX on the dendrimer compared to bare dendrimer. Interestingly, cell cytotoxicity and DAPI staining results displayed lower cell cytotoxicity and apoptosis for MCF-7 cells that were treated with dendrimer-MTX compared to free MTX. Conclusion: The obtained data indicated that dendrimer-MTX complex had a higher cellular uptake, but cell cytotoxicity and apoptosis were lower than free MTX.

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APA: Copy

Abedi Gaballu, Fereydoon, MANSOORI, BEHZAD, & DEHGHAN, GHOLAMREZA. (2019). EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 30(5 ), 381-392. SID. https://sid.ir/paper/962343/en

Vancouver: Copy

Abedi Gaballu Fereydoon, MANSOORI BEHZAD, DEHGHAN GHOLAMREZA. EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2019;30(5 ):381-392. Available from: https://sid.ir/paper/962343/en

IEEE: Copy

Fereydoon Abedi Gaballu, BEHZAD MANSOORI, and GHOLAMREZA DEHGHAN, “EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 30, no. 5 , pp. 381–392, 2019, [Online]. Available: https://sid.ir/paper/962343/en

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