The effect of sodium butyrate as a histone deacetylase inhibitor on the gene expression of Bid in HT-29 human colorectal cancer cell line

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Amiri Najmeh; FOROUZESH FLORA; NAZEMALHOSSEINI MOJARAD EHSAN; SHABANI MAHDI

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Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2020 | Volume:30 | Issue:1 Page(s): 40-50

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Title

The effect of sodium butyrate as a histone deacetylase inhibitor on the gene expression of Bid in HT-29 human colorectal cancer cell line

Author(s)

Amiri Najmeh | FOROUZESH FLORA | NAZEMALHOSSEINI MOJARAD EHSAN | SHABANI MAHDI | Issue Writer Certificate

Keywords

Colorectal cancer

Bid gene

Sodium butyrate

HT-29

Abstract

Background: Sodium butyrate (NaBr), a histone deacetylase inhibitor, has emerged as a promising anticancer drug for multiple cancers. The aim of this study was to investigate the effect of Sodium butyrate on the changes in the expression of the Bid gene in human Colorectal cancer cell line. Materials and methods: HT-29 cell line was cultured. Cells were treated with different concentration of NaBr (from 6. 25 mM to 200mM) for 24, 48 and 72 hours. Cytotoxicity and IC50 were evaluated by MTT assay. The effect of NaBr on Bid gene expression was examined by PCR and qRT-PCR. Results: The IC50 for 24, 48, and 72 hours were 95mM, 25mM and, 12. 5mM respectively. Antiproliferative activity of NaBr was dependent on time and dose manner. The results of real time PCR showed that the expression of Bid gene in treated cells with IC50 concentration increased significantly (p<0. 05) in comparison with the control group. Conclusion: The results showed that NaBr inhibited the growth and induced apoptosis in the HT-29 cell line which can be due to the increase in Bid gene expression. So, the change in expression of the apoptosis genes can effect by histone deacetylase inhibitors.

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APA: Copy

Amiri, Najmeh, FOROUZESH, FLORA, NAZEMALHOSSEINI MOJARAD, EHSAN, & SHABANI, MAHDI. (2020). The effect of sodium butyrate as a histone deacetylase inhibitor on the gene expression of Bid in HT-29 human colorectal cancer cell line. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 30(1 ), 40-50. SID. https://sid.ir/paper/963428/en

Vancouver: Copy

Amiri Najmeh, FOROUZESH FLORA, NAZEMALHOSSEINI MOJARAD EHSAN, SHABANI MAHDI. The effect of sodium butyrate as a histone deacetylase inhibitor on the gene expression of Bid in HT-29 human colorectal cancer cell line. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2020;30(1 ):40-50. Available from: https://sid.ir/paper/963428/en

IEEE: Copy

Najmeh Amiri, FLORA FOROUZESH, EHSAN NAZEMALHOSSEINI MOJARAD, and MAHDI SHABANI, “The effect of sodium butyrate as a histone deacetylase inhibitor on the gene expression of Bid in HT-29 human colorectal cancer cell line,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 30, no. 1 , pp. 40–50, 2020, [Online]. Available: https://sid.ir/paper/963428/en

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