EFFECTS OF DIFFERENT EGG YOLK CONCENTRATIONS ON THE MOTILITY AND VIABILITY OF MARKHOZ GOAT SPERM AFTER FREEZING STEP OF CRYOPRESERVATION AND THAWING

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FARSHAD A.; FAZELI P.; KHALILI B.; AKHOUNDZADEH S.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2008 | Volume:15 | Issue:2 Page(s): 257-260

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Title

EFFECTS OF DIFFERENT EGG YOLK CONCENTRATIONS ON THE MOTILITY AND VIABILITY OF MARKHOZ GOAT SPERM AFTER FREEZING STEP OF CRYOPRESERVATION AND THAWING

Author(s)

FARSHAD A. | FAZELI P. | KHALILI B. | AKHOUNDZADEH S. | Issue Writer Certificate

Keywords

CRYOPRESERVATIONQ3

EGG YOLKQ3

MARKHOZ GOATQ3

SPERMQ3

Abstract

The effect of concentrations of EGG YOLK (2.5, 5, 7.5, 10, 15 and 20%) in Tris diluent on viability, motility and progressive motility of frozen-thawed MARKHOZ GOAT in breeding season was studied. The semen was collected from 5 Markhoz bucks twice a week during 7 weeks. Every time after collecting Fresh semen evaluated for motility and SPERM concentration. Semen diluted with Tris-Citric acid- Fructose (TCF) extender containing different concentration EGG YOLK was cooled to 5oc and then frozen at-80oc in nitrogen vapor and stored it in liquid nitrogen. Thawing was performed by placing the straws in a water bath at 37oC for 30s. The post-thaw viability was 57.48, 60.17, 55.21, 54.91, 53.74 and 49.53% for 2.5, 5, 7.5, 10, 15 and 20% of EGG YOLK concentration, respectively. The effects of different concentrations of EGG YOLK on post-thaw motility were as 52.92, 55.71, 50.78, 49.85, 49.21 and 45.35%, respectively. The results for post-thaw progressive motility showed 39.21, 42.28, 37.85, 36, 37 and 32.42% in different concentrations of EGG YOLK, respectively. Maximum and minimum of viability, motility and progressive motility were obtained in 5 and 20% EGG YOLK concentration, respectively. The result show a significant decrease in SPERM viability and motility by increasing the EGG YOLK in Tris buffer (P<0.05). Therefore, using 5% of EGG YOLK concentration in Tris diluent can be suitable for CRYOPRESERVATION SPERM MARKHOZ GOAT during breeding season.

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APA: Copy

FARSHAD, A., FAZELI, P., KHALILI, B., & AKHOUNDZADEH, S.. (2008). EFFECTS OF DIFFERENT EGG YOLK CONCENTRATIONS ON THE MOTILITY AND VIABILITY OF MARKHOZ GOAT SPERM AFTER FREEZING STEP OF CRYOPRESERVATION AND THAWING. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 15(2), 257-260. SID. https://sid.ir/paper/9641/en

Vancouver: Copy

FARSHAD A., FAZELI P., KHALILI B., AKHOUNDZADEH S.. EFFECTS OF DIFFERENT EGG YOLK CONCENTRATIONS ON THE MOTILITY AND VIABILITY OF MARKHOZ GOAT SPERM AFTER FREEZING STEP OF CRYOPRESERVATION AND THAWING. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2008;15(2):257-260. Available from: https://sid.ir/paper/9641/en

IEEE: Copy

A. FARSHAD, P. FAZELI, B. KHALILI, and S. AKHOUNDZADEH, “EFFECTS OF DIFFERENT EGG YOLK CONCENTRATIONS ON THE MOTILITY AND VIABILITY OF MARKHOZ GOAT SPERM AFTER FREEZING STEP OF CRYOPRESERVATION AND THAWING,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 15, no. 2, pp. 257–260, 2008, [Online]. Available: https://sid.ir/paper/9641/en

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