INVESTIGATION OF DIFFERENT GLYCEROL AND EGG YOLK CONCENTRATION ON FREEZING BAKHTIARI RAM SEMEN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FOROUZANFAR MOHSEN; FAZILATI MOHAMMAD; HOSSEINI SEYED MORTEZA; MOULAVI FARIBA; HAJIAN MAHDI; ASAD ELAH SALEHI S.; RABIEI ASAD ELAH; NASRESFAHANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Anatomical Sciences Journal Year:2007 | Volume:5 | Issue:18 Page(s): 17-25

Download Full-Text

Persian Verion

View:

911

Download:

Cites:

Information Journal Paper

Title

INVESTIGATION OF DIFFERENT GLYCEROL AND EGG YOLK CONCENTRATION ON FREEZING BAKHTIARI RAM SEMEN

Author(s)

Keywords

RAMQ3

SEMENQ3

GLYCEROLQ4

FREEZING AND THAWINGQ3

Abstract

Purpose: GLYCEROL has been the most widely used cryopreservation agent for spermatozoa and a wide range of factors affects its action on sperm viability and fertilizing capacity. Moreover egg yolk is a common constituent of SEMEN diluents, protects the spermatozoa against cold shock and confers protection during FREEZING AND THAWING .The aim of this study was to investigate of different GLYCEROL and egg yolk concentration for optimum freezing of RAM SEMEN.Materials and Methods: Nine SEMEN extender with different concentration of egg yolk (10%, 15%, 20%) and GLYCEROL (4%, 6%, 8%) in a Tris-based media were tested for cryopreservation of SEMEN. SEMEN was collected from Bakhtiari RAMs by an artificial vagina (38-40°C) two consecutive SEMEN collections from each RAM were pooled. The ejaculates were diluted with each extender, cooled to 5°C and left for 2 hours. Thereafter, it was packed in 0.5 ml straws (final cellular concentration 100-200 ×106 spz/ml). Then the frozen straws in nitrogen vapors (5 cm above nitrogen liquid). the straws were then stored in liquid nitrogen until further use.Results: The results showed that frozen sperm in all treatment groups had motility after thawing but the percentage of motility in different groups are different.Conclusion: In conclusion the results of present study indicate that the 20% egg yolk – Tris – 8% GLYCEROL extender was significantly superior to other extender, in protecting the sperm during FREEZING AND THAWING and saving the motility of spermatozoa. While the 10% egg yolk – Tris – 4% GLYCEROL show the lowest motility after freezing thawing.

Cites

No record.

References

No record.

Cite

APA: Copy

FOROUZANFAR, MOHSEN, FAZILATI, MOHAMMAD, HOSSEINI, SEYED MORTEZA, MOULAVI, FARIBA, HAJIAN, MAHDI, ASAD ELAH SALEHI, S., RABIEI, ASAD ELAH, & NASRESFAHANI, MOHAMMAD HOSSEIN. (2007). INVESTIGATION OF DIFFERENT GLYCEROL AND EGG YOLK CONCENTRATION ON FREEZING BAKHTIARI RAM SEMEN. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 5(18), 17-25. SID. https://sid.ir/paper/81481/en

Vancouver: Copy

FOROUZANFAR MOHSEN, FAZILATI MOHAMMAD, HOSSEINI SEYED MORTEZA, MOULAVI FARIBA, HAJIAN MAHDI, ASAD ELAH SALEHI S., RABIEI ASAD ELAH, NASRESFAHANI MOHAMMAD HOSSEIN. INVESTIGATION OF DIFFERENT GLYCEROL AND EGG YOLK CONCENTRATION ON FREEZING BAKHTIARI RAM SEMEN. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2007;5(18):17-25. Available from: https://sid.ir/paper/81481/en

IEEE: Copy

MOHSEN FOROUZANFAR, MOHAMMAD FAZILATI, SEYED MORTEZA HOSSEINI, FARIBA MOULAVI, MAHDI HAJIAN, S. ASAD ELAH SALEHI, ASAD ELAH RABIEI, and MOHAMMAD HOSSEIN NASRESFAHANI, “INVESTIGATION OF DIFFERENT GLYCEROL AND EGG YOLK CONCENTRATION ON FREEZING BAKHTIARI RAM SEMEN,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 5, no. 18, pp. 17–25, 2007, [Online]. Available: https://sid.ir/paper/81481/en

Related Journal Papers