Methicillin Resistant Staphylococcus aureus and Vancomycin Resistant Enterococci in Hospital Sewage in Isfahan

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ZARKESH ESFAHANI FATEMEH SADAT; Amin Nasrin; RAHIMI FATEH

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2020 | Volume:25 | Issue:90 Page(s): 18-26

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Title

Methicillin Resistant Staphylococcus aureus and Vancomycin Resistant Enterococci in Hospital Sewage in Isfahan

Author(s)

ZARKESH ESFAHANI FATEMEH SADAT | Amin Nasrin | RAHIMI FATEH | Issue Writer Certificate

Keywords

MRSA

VRE

hospital sewage

Isfahan

Abstract

Background and objective: Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) cause a wide range of infections in the hospital and community. Hospital and municipal wastewater are the most important reservoirs of these bacteria, and inefficiency of wastewater treatment systems results in the entrance of these bacteria into groundwater, surface water and drinking water of society. The aim of this study was to study the presence of MRSA and VRE isolates in a hospital sewage in Isfahan, Iran. Materials and methods: Sampling was carried out 2 times during spring and summer 2018 from a hospital sewage in Isfahan. Samples were diluted and 100 microliters were spread on Baird-parker agar supplemented with 2 µ g/ml oxacillin and m-Enterococcus agar supplemented with 4 µ g/ml vancomycin and inoculated plates were incubated for 48 h at 37° C. All black colonies with halo on Baird-parker agar and red colonies on m-Enterococcus agar were collected and identified at the species level using PCR by the species specific primers. The resistance of all isolated colonies from Baird-parker agar and m-Enterococcus agar to cefoxitin and vancomycin, respectively, was tested using disk diffusion method by the guidelines of the Clinical & Laboratory Standards Institute (CLSI). Results: A total of 185 and 110 colonies were collected from Baird-parker agar and m-Enterococcus agar plates, respectively. The results of the PCR tests revealed that none of the black colonies on Baird-parker agar were S. aureus and only 3 (3%) vancomycin resistant Enterococcus faecium strains were identified among all red colonies on m-Enterococcus agar plates. Conclusion: The results of this study revealed the absence of MRSA strains and relatively low presence of VRE in the effluent of the hospital in Isfahan, which indicating the high quality and efficiency of the hospital disinfection system for complete removal of MRSA strains and relatively high elimination of VRE strains from hospital wastewater and prevent them from entering municipal wastewater. It seems that this system is not able to eliminate other bacteria such as coagulase-negative staphylococci, Leuconostoc and Lactococcus, which could be a serious threat to public health.

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APA: Copy

ZARKESH ESFAHANI, FATEMEH SADAT, Amin, Nasrin, & RAHIMI, FATEH. (2020). Methicillin Resistant Staphylococcus aureus and Vancomycin Resistant Enterococci in Hospital Sewage in Isfahan. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 25(90 ), 18-26. SID. https://sid.ir/paper/966526/en

Vancouver: Copy

ZARKESH ESFAHANI FATEMEH SADAT, Amin Nasrin, RAHIMI FATEH. Methicillin Resistant Staphylococcus aureus and Vancomycin Resistant Enterococci in Hospital Sewage in Isfahan. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2020;25(90 ):18-26. Available from: https://sid.ir/paper/966526/en

IEEE: Copy

FATEMEH SADAT ZARKESH ESFAHANI, Nasrin Amin, and FATEH RAHIMI, “Methicillin Resistant Staphylococcus aureus and Vancomycin Resistant Enterococci in Hospital Sewage in Isfahan,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 25, no. 90 , pp. 18–26, 2020, [Online]. Available: https://sid.ir/paper/966526/en

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