THE EFFECTS OF FIG LEAF EXTRACT ON THE MATRIX METALLOPROTEINASES (MMPS) ACTIVITIES IN THE FIBROBLAST (HEP2) CELL CULTURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ATYABI N.; NIKNAM G.; NASIRI S.M.; TAHERI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2017 | Volume:13 | Issue:1 Page(s): 13-25

Download Full-Text

Persian Verion

View:

1,101

Download:

Cites:

Information Journal Paper

Title

THE EFFECTS OF FIG LEAF EXTRACT ON THE MATRIX METALLOPROTEINASES (MMPS) ACTIVITIES IN THE FIBROBLAST (HEP2) CELL CULTURE

Author(s)

ATYABI N. | NIKNAM G. | NASIRI S.M. | TAHERI M. | Issue Writer Certificate

Keywords

MATRIX METALLOPROTEINASESQ1

FIBROBLASTQ1

FIG LEAF EXTRACTSQ1

ZYMOGRAPHYQ1

CYTOTOXICITYQ1

Abstract

The aim of this study was to evaluate the effects of fig leaf extract on the activity of MMPs, in FIBROBLAST (HEP2) cell culture. For this purpose, fig leaf extract was prepared in three forms: 1) aqueous, 2) hydro-alcoholic and 3) alcoholic extracts. Using a 24 wells cell culture plates, six wells of a plate were filled with 5ml aqueous extract, 10ml aqueous extract, 5ml hydro _ alcoholic extract, 10ml hydro_ alcoholic extract, 5ml alcoholic extract, and 1ml alcohlic extract, as test samples, respectively. For each test sample, it was considered a control well, with the same content as test, but without the extracts. Thereafter, the plates were incubated at 37° C and 5%CO2. The effects of different extracts, were investigated after 24, 48 and 72 hours, using an inverted microscope and determination of cell viability and CYTOTOXICITY by trypan blue staining. and ZYMOGRAPHY test with gelatin for MMPs Activities.The result of the study were as follows: the alcoholic extract at a concentration of 5ml caused cell death after 24 h, and a concentration of 1ml of alcoholic extract, also led to cell death after 48 hours. Hydro_alcoholic extract caused cell death, at a concentration of 10ml, after 72 hours. In ZYMOGRAPHY test, it was observed the inhibitory effect of fig leaf extract on the MMPs activities, in all tested doses. However, there were seen enzyme activities in all control tests which showed the same molecular weight bands, 92KD, as was shown in standard marker and belonged to MMP9. The present study, confirmed the inhibitory effect of fig leaf extract on the activity of MMPs in cell cultures. It is recommended to continue this study with molecular methods besides ZYMOGRAPHY, and clinical observation, using the fig leaf extract and its inhibitory effects on the other kinds of MMPs, separately.

Cites

No record.

References

No record.

Cite

APA: Copy

ATYABI, N., NIKNAM, G., NASIRI, S.M., & TAHERI, M.. (2017). THE EFFECTS OF FIG LEAF EXTRACT ON THE MATRIX METALLOPROTEINASES (MMPS) ACTIVITIES IN THE FIBROBLAST (HEP2) CELL CULTURE. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 13(1), 13-25. SID. https://sid.ir/paper/97411/en

Vancouver: Copy

ATYABI N., NIKNAM G., NASIRI S.M., TAHERI M.. THE EFFECTS OF FIG LEAF EXTRACT ON THE MATRIX METALLOPROTEINASES (MMPS) ACTIVITIES IN THE FIBROBLAST (HEP2) CELL CULTURE. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2017;13(1):13-25. Available from: https://sid.ir/paper/97411/en

IEEE: Copy

N. ATYABI, G. NIKNAM, S.M. NASIRI, and M. TAHERI, “THE EFFECTS OF FIG LEAF EXTRACT ON THE MATRIX METALLOPROTEINASES (MMPS) ACTIVITIES IN THE FIBROBLAST (HEP2) CELL CULTURE,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 13, no. 1, pp. 13–25, 2017, [Online]. Available: https://sid.ir/paper/97411/en

Related Journal Papers