EVALUATION OF MATRIX METALOPROTEINASE 2 (MMP2) ACTIVITY IN CONTACT NICKEL DERMATITIS USING ZYMOANALYSIS IN COMPARISON TO NORMAL INDIVIDUALS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FALAK REZA; KHORAMIZADEH MOHAMMAD REZA; PEZESHKI M.; MANSOURI P.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Qom University of Medical Sciences Journal Year:2008 | Volume:1 | Issue:4 Page(s): 45-52

Download Full-Text

Persian Verion

View:

1,353

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF MATRIX METALOPROTEINASE 2 (MMP2) ACTIVITY IN CONTACT NICKEL DERMATITIS USING ZYMOANALYSIS IN COMPARISON TO NORMAL INDIVIDUALS

Author(s)

FALAK REZA | KHORAMIZADEH MOHAMMAD REZA | PEZESHKI M. | MANSOURI P. | Issue Writer Certificate

Keywords

NICKEL DERMATITISQ3

MATRIX METALOPROTEINASE 2 (MMP2)Q3

FIBROBLASTQ3

ZYMOANALYSISQ3

Abstract

Background and objective: Matrix Metaloproteinases (MMPs), produced mainly by FIBROBLASTs, play a major role in wound healing processes. This study aimed to investigate the MMP2 activity in dermal FIBROBLASTs found in chronic contact NICKEL DERMATITIS lesions.Methods: In order to study the role of MMP2 in contact NICKEL DERMATITIS, FIBROBLAST from patients and healthy individuals were cultured based on ex-plantation of skin. MMP2 activity was measured in FIBROBLAST culture media using ZYMOANALYSIS. Zymograms were then analyzed by quantitative densitometry. MTT assay was also used to evaluate and compare proliferation capacity of FIBROBLASTs in both cell cultures.Results: The mean MMP2 activity 6-8 days after ex-plantation was significantly higher in patients FIBROBLASTs than in normal individuals (170±9.2 and 134.3±5.9 in patients and normals, respectively (p<0.05)). The proliferation capacity of patients' FIBROBLASTs was significantly higher than that of normal cases (385938±2816 and 270261±6527 respectively, p<0.05) in the same days.Conclusion: Our study shows a significant increase in degenerative activity of FIBROBLASTs in NICKEL DERMATITIS lesions. It was also seen that the MMP2 activity per cell was significantly higher in patients' FIBROBLASTs compared to healthy individuals and that gelatinolytic activity of MMP2 is independent of cell number. Therefore, intra- and inter-cellular signals may be altered in FIBROBLASTs of NICKEL DERMATITIS lesions which lead to promotion of FIBROBLAST response to mitogenic and fibrogenic stimulations.

Cites

No record.

References

No record.

Cite

APA: Copy

FALAK, REZA, KHORAMIZADEH, MOHAMMAD REZA, PEZESHKI, M., & MANSOURI, P.. (2008). EVALUATION OF MATRIX METALOPROTEINASE 2 (MMP2) ACTIVITY IN CONTACT NICKEL DERMATITIS USING ZYMOANALYSIS IN COMPARISON TO NORMAL INDIVIDUALS. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 1(4), 45-52. SID. https://sid.ir/paper/132488/en

Vancouver: Copy

FALAK REZA, KHORAMIZADEH MOHAMMAD REZA, PEZESHKI M., MANSOURI P.. EVALUATION OF MATRIX METALOPROTEINASE 2 (MMP2) ACTIVITY IN CONTACT NICKEL DERMATITIS USING ZYMOANALYSIS IN COMPARISON TO NORMAL INDIVIDUALS. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2008;1(4):45-52. Available from: https://sid.ir/paper/132488/en

IEEE: Copy

REZA FALAK, MOHAMMAD REZA KHORAMIZADEH, M. PEZESHKI, and P. MANSOURI, “EVALUATION OF MATRIX METALOPROTEINASE 2 (MMP2) ACTIVITY IN CONTACT NICKEL DERMATITIS USING ZYMOANALYSIS IN COMPARISON TO NORMAL INDIVIDUALS,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 1, no. 4, pp. 45–52, 2008, [Online]. Available: https://sid.ir/paper/132488/en

Related Journal Papers