Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers

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Ahmed Mohammed Rehab; Mohamed Shawky Heba; Ahmed Rashed Laila; Mohamed Elhanbuli Hala; Nabil Abdelhafez Dalia; Sayed Said Eman; Sayed Said Eman; Mostafa Shamardan Ramadan; Hosny Mahmoud Rania

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2021 | Volume:24 | Issue:12 Page(s): 1753-1762

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Title

Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers

Author(s)

Keywords

Bile duct ligation

Hydrogen sulfide

Liver fibrosis

Mesenchymal stem cells

Abstract

Objective(s): Liver fibrosis eventually develops into cirrhosis and hepatic failure, which can only be treated with liver transplantation. We aimed to assess the potential role of Hydrogen sulfide (H2S) alone and combined with bone marrow-derived Mesenchymal stem cells (BM-MSCs) on hepatic fibrosis induced by bile-duct ligation (BDL) and to compare their effects to silymarin. Materials and Methods: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB), and alkaline phosphatase (ALP) were investigated in serum. Gene expression levels of CBS (cystathionine β-synthase), CSE (cystathionine γ-lyase), and alpha-smooth muscle actin (α-SMA) were measured in liver tissues using RT-PCR. Hepatic protein kinase (Akt) was assessed by Western blot assay. Liver oxidative stress markers, malondialdehyde (MDA), and reduced glutathione (GSH) were analyzed by the colorimetric method. Lipocalin-2 (LCN2) and transforming growth factor-β (TGF-β ) were measured using ELIZA. Liver tissues were examined by H&E and Masson trichome staining for detection of liver necrosis or fibrosis. Caspase 3 expression was evaluated by immunohistochemistry. Results: H2S and BM-MSCs ameliorated liver function and inhibited inflammation and oxidative stress detected by significantly decreased serum ALT, AST, ALP, TB, and hepatic MDA, Akt, TGF-β , LCN2, and α-SMA expression and significantly increased CBS and CSE gene expression levels. They attenuated hepatic apoptosis evidenced by decreased hepatic caspase expression. Conclusion: Combined treatment with H2S and BM-MSCs could attenuate Liver fibrosis induced by BDL through mechanisms such as anti-inflammation, anti-oxidation, anti-apoptosis, anti-fibrosis, and regenerative properties indicating that using H2S and MSCs may represent a promising approach for management of cholestatic Liver fibrosis.

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APA: Copy

Ahmed Mohammed, Rehab, Mohamed Shawky, Heba, Ahmed Rashed, Laila, Mohamed Elhanbuli, Hala, Nabil Abdelhafez, Dalia, Sayed Said, Eman, Mostafa Shamardan, Ramadan, Hosny Mahmoud, Rania, & Sayed Said, Eman. (2021). Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 24(12), 1753-1762. SID. https://sid.ir/paper/976278/en

Vancouver: Copy

Ahmed Mohammed Rehab, Mohamed Shawky Heba, Ahmed Rashed Laila, Mohamed Elhanbuli Hala, Nabil Abdelhafez Dalia, Sayed Said Eman, Mostafa Shamardan Ramadan, Hosny Mahmoud Rania, Sayed Said Eman. Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2021;24(12):1753-1762. Available from: https://sid.ir/paper/976278/en

IEEE: Copy

Rehab Ahmed Mohammed, Heba Mohamed Shawky, Laila Ahmed Rashed, Hala Mohamed Elhanbuli, Dalia Nabil Abdelhafez, Eman Sayed Said, Ramadan Mostafa Shamardan, Rania Hosny Mahmoud, and Eman Sayed Said, “Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 24, no. 12, pp. 1753–1762, 2021, [Online]. Available: https://sid.ir/paper/976278/en

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