The Effect of Secretome, Xenogenic Bone MarrowDerived Mesenchymal Stem Cells, Bone Morphogenetic Protein-2, Hydroxyapatite Granule and Mechanical Fixation in Critical-Size Defects of Rat Models

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Dilogo Ismail Hadisoebroto; Fiolin Jessica; Feby Canintika Annisa; Pawitan Jeanne Adiwinata; Luviah Evah

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Journal Paper

Paper Information

Journal: Archives of Bone and Joint Surgery Year:2022 | Volume:10 | Issue:1 Page(s): 17-22

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Title

The Effect of Secretome, Xenogenic Bone MarrowDerived Mesenchymal Stem Cells, Bone Morphogenetic Protein-2, Hydroxyapatite Granule and Mechanical Fixation in Critical-Size Defects of Rat Models

Author(s)

Keywords

Secretome

Mesenchymal Stem Cell

Radiographic

Histomorphometric

Bone healing

Sprague Dawley Rat

Abstract

Background: Recent studies have shown that human bone marrow-derived Mesenchymal Stem Cells (hBM-MSCs) have several drawbacks in treating critical-sized bone defect (CSD). Secretome may offer considerable advantages over living cells in terms of potency, manufacturing and storing easiness, and potential as a ready-to-go osteoinductive agent. However, thus far, there are no studies regarding the efficacy of Secretome in Bone healing. The objective of this study is to investigate the effect of the Secretome in rat models with CSD. Methods: This was an experimental study with post-test only control group design using 60 skeletally mature Sprague Dawley Rat which was divided evenly into 5 treatment groups (MSC only, Secretome only, MSC + Secretome, MSC + Secretome + BMP-2, Control group using Normal Saline). We used Bone Marrow derived MSC in this research. The critical-sized bone defect was created by performing osteotomy and defect was treated according to the groups. Rats were sacrificed on 2nd and 4th week and we measured the radiological outcome using Radiographic Union Score for Tibia (RUST) and Histomorphometric (callus, osseous, cartilage, fibrous, and void area) evaluation using Image J. Results: There was no difference in the weight of rats between groups before and after the intervention. RUST score in all intervention group is significantly higher than the control group, however, the MSC-only group was not statistically significant higher than the control group. There is no statistically significant difference in RUST Score between intervention groups. Histomorphometric evaluation showed that total callus formation is the widest in the MSC+Secretome+BMP-2 combination group while the osseous area is found highest on the Secretome-only group. Conclusion: Secretome, whether used solely or combined with BM-MSC and BMP-2, is a novel, potent bone-healing agent for CSD in rat models.

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APA: Copy

Fiolin, Jessica, Feby, Canintika Annisa, & Luviah, Evah. (2022). The Effect of Secretome, Xenogenic Bone MarrowDerived Mesenchymal Stem Cells, Bone Morphogenetic Protein-2, Hydroxyapatite Granule and Mechanical Fixation in Critical-Size Defects of Rat Models. THE ARCHIVES OF BONE AND JOINT SURGERY, 10(1), 17-22. SID. https://sid.ir/paper/983805/en

Vancouver: Copy

Fiolin Jessica, Feby Canintika Annisa, Luviah Evah. The Effect of Secretome, Xenogenic Bone MarrowDerived Mesenchymal Stem Cells, Bone Morphogenetic Protein-2, Hydroxyapatite Granule and Mechanical Fixation in Critical-Size Defects of Rat Models. THE ARCHIVES OF BONE AND JOINT SURGERY[Internet]. 2022;10(1):17-22. Available from: https://sid.ir/paper/983805/en

IEEE: Copy

Jessica Fiolin, Canintika Annisa Feby, and Evah Luviah, “The Effect of Secretome, Xenogenic Bone MarrowDerived Mesenchymal Stem Cells, Bone Morphogenetic Protein-2, Hydroxyapatite Granule and Mechanical Fixation in Critical-Size Defects of Rat Models,” THE ARCHIVES OF BONE AND JOINT SURGERY, vol. 10, no. 1, pp. 17–22, 2022, [Online]. Available: https://sid.ir/paper/983805/en

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