EVALUATION OF MYCN AMPLIFICATION IN IRANIAN PATIENTS WITH NEUROBLASTOMA BY CONVENTIONAL AND REAL TIME PCR

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POOPAK BEHZAD; ARZANIAN MOHAMMAD TAGHI; VOSOUGH PARVANEH; GHARIB ATOOSA; HAMIDIEH AMIR ALI; EHSANI MOHAMMAD; PARSANIA MASOOD; YOUSEFIAN ABOLFAZL; JAHANGIRPOUR MOHAMMAD ALI; FARAHANI KOBRA

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Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2011 | Volume:21 | Issue:3 (65) Page(s): 188-195

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Title

EVALUATION OF MYCN AMPLIFICATION IN IRANIAN PATIENTS WITH NEUROBLASTOMA BY CONVENTIONAL AND REAL TIME PCR

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Keywords

MYCNQ3

AMPLIFICATIONQ2

PCRQ2

NEUROBLASTOMAQ2

Abstract

Background: There is an international consensus that evaluation of MYCNamplification should be done in all cases of newly diagnosed NEUROBLASTOMA. MYCN is the most important prognostic factor for NEUROBLASTOMA and determines treatment strategy.Materials and methods: In this study we evaluated paraffin embedded tissue block and bone marrow aspiration of 75 NEUROBLASTOMA patients with mean age of 4.1 years, including 32 female and 43 male, by conventional and also real time quantitative PCR.Results: Forty eight and 43 percent were MYCN AMPLIFICATION positive by Real time and conventional PCR, respectively. 28% of patients less than one year old and 48% of patients older than one year showed MYCN AMPLIFICATION.50 percent of cases with pathological diagnosis of small round cell tumor had MYCN AMPLIFICATION.Conclusion: PCR is a fast, reliable and cost effective method for the evaluation of MYCN AMPLIFICATION and can be performed using DNA extracted from small tissue samples and paraffin embedded blocks. As expected regarding detection power and convenience, Real time PCR was superior to conventional PCR.

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APA: Copy

POOPAK, BEHZAD, ARZANIAN, MOHAMMAD TAGHI, VOSOUGH, PARVANEH, GHARIB, ATOOSA, HAMIDIEH, AMIR ALI, EHSANI, MOHAMMAD, PARSANIA, MASOOD, YOUSEFIAN, ABOLFAZL, JAHANGIRPOUR, MOHAMMAD ALI, & FARAHANI, KOBRA. (2011). EVALUATION OF MYCN AMPLIFICATION IN IRANIAN PATIENTS WITH NEUROBLASTOMA BY CONVENTIONAL AND REAL TIME PCR. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 21(3 (65)), 188-195. SID. https://sid.ir/paper/98515/en

Vancouver: Copy

POOPAK BEHZAD, ARZANIAN MOHAMMAD TAGHI, VOSOUGH PARVANEH, GHARIB ATOOSA, HAMIDIEH AMIR ALI, EHSANI MOHAMMAD, PARSANIA MASOOD, YOUSEFIAN ABOLFAZL, JAHANGIRPOUR MOHAMMAD ALI, FARAHANI KOBRA. EVALUATION OF MYCN AMPLIFICATION IN IRANIAN PATIENTS WITH NEUROBLASTOMA BY CONVENTIONAL AND REAL TIME PCR. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2011;21(3 (65)):188-195. Available from: https://sid.ir/paper/98515/en

IEEE: Copy

BEHZAD POOPAK, MOHAMMAD TAGHI ARZANIAN, PARVANEH VOSOUGH, ATOOSA GHARIB, AMIR ALI HAMIDIEH, MOHAMMAD EHSANI, MASOOD PARSANIA, ABOLFAZL YOUSEFIAN, MOHAMMAD ALI JAHANGIRPOUR, and KOBRA FARAHANI, “EVALUATION OF MYCN AMPLIFICATION IN IRANIAN PATIENTS WITH NEUROBLASTOMA BY CONVENTIONAL AND REAL TIME PCR,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 21, no. 3 (65), pp. 188–195, 2011, [Online]. Available: https://sid.ir/paper/98515/en

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