DETECTION OF ‎ ‎ CMLA/TETR, ‎BLA PSE-1, BLA TEM AND SIP B IN THE ‎SALMONELLA STRAINS BY MULTIPLEX-PCR METHOD AND THEIR ANTIBIOTIC ‎RESISTANCE PATTERN

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NAJAFI MOHAMMAD REZA; PARVIZ MEHDI; AMINI KUMARSS

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Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2017 | Volume:27 | Issue:2 Page(s): 119-125

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Title

DETECTION OF ‎ ‎ CMLA/TETR, ‎BLA PSE-1, BLA TEM AND SIP B IN THE ‎SALMONELLA STRAINS BY MULTIPLEX-PCR METHOD AND THEIR ANTIBIOTIC ‎RESISTANCE PATTERN

Author(s)

NAJAFI MOHAMMAD REZA | PARVIZ MEHDI | AMINI KUMARSS | Issue Writer Certificate

Keywords

CMLA-TETRQ1

PSE-1Q1

TEMQ1

SIP BQ2

SALMONELLAQ1

MULTIPLEX-PCRQ1

Abstract

Background: Gastroenteritis due to SALMONELLA is common in human and considered as a global ‎dilemma of public health. This study was done to determine cmlA/tetR, ‎bla PSE-1, bla TEM and SIP B ‎in the SALMONELLA strains by MULTIPLEX-PCR method and their antibiotic susceptibility profile.Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from ‎patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined ‎using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for ‎determination of these target genes by the specific oligonucleotides primers.Results: Of 163 collected samples, 48(29.4%) SALMONELLA spp., were obtained, which 25(52.1%) were ‎S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed ‎that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and ‎chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, ‎gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of ‎Salmonella spp., isolates carried cml/tetR and sipB genes, respectively.Conclusion: According to our results, detection and genotyping of virulence genes and comparison ‎with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial ‎purposes.‎ Keywords: cmlA/tetR, PSE-1, TEM, SIP B, SALMONELLA, MULTIPLEX-PCR.

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APA: Copy

NAJAFI, MOHAMMAD REZA, PARVIZ, MEHDI, & AMINI, KUMARSS. (2017). DETECTION OF ‎‌‎ CMLA/TETR, ‎BLA PSE-1, BLA TEM AND SIP B IN THE ‎SALMONELLA STRAINS BY MULTIPLEX-PCR METHOD AND THEIR ANTIBIOTIC ‎RESISTANCE PATTERN. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 27(2), 119-125. SID. https://sid.ir/paper/98583/en

Vancouver: Copy

NAJAFI MOHAMMAD REZA, PARVIZ MEHDI, AMINI KUMARSS. DETECTION OF ‎‌‎ CMLA/TETR, ‎BLA PSE-1, BLA TEM AND SIP B IN THE ‎SALMONELLA STRAINS BY MULTIPLEX-PCR METHOD AND THEIR ANTIBIOTIC ‎RESISTANCE PATTERN. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2017;27(2):119-125. Available from: https://sid.ir/paper/98583/en

IEEE: Copy

MOHAMMAD REZA NAJAFI, MEHDI PARVIZ, and KUMARSS AMINI, “DETECTION OF ‎‌‎ CMLA/TETR, ‎BLA PSE-1, BLA TEM AND SIP B IN THE ‎SALMONELLA STRAINS BY MULTIPLEX-PCR METHOD AND THEIR ANTIBIOTIC ‎RESISTANCE PATTERN,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 27, no. 2, pp. 119–125, 2017, [Online]. Available: https://sid.ir/paper/98583/en

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