DIFFERENTIATION POTENTIAL OF HUMAN CD133 AND CD34 POSITIVE HEMATOPOIETIC STEM CELLS INTO MOTOR NEURON-LIKE CELLS; AN IN VITRO STUDY

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ALAVI MOGHADDAM SEPIDEH; FAGHIHI FAEZEH; HAYATI ROODBARI NASIM

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2018 | Volume:28 | Issue:2 Page(s): 92-103

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Title

DIFFERENTIATION POTENTIAL OF HUMAN CD133 AND CD34 POSITIVE HEMATOPOIETIC STEM CELLS INTO MOTOR NEURON-LIKE CELLS; AN IN VITRO STUDY

Author(s)

ALAVI MOGHADDAM SEPIDEH | FAGHIHI FAEZEH | HAYATI ROODBARI NASIM | Issue Writer Certificate

Keywords

CD133++Q2

CD34++Q1

HEMATOPOIETIC STEM CELLQ1

MOTOR NEURONQ2

UMBILICAL CORD BLOODQ2

Abstract

Background: We were studied on differentiation potential of CD133++ and CD34++ HEMATOPOIETIC STEM CELLs into MOTOR NEURON like cells. Materials and methods: CD133++ and CD34++ HSCs were isolated from human UCB using MACS system. After cell characterization using flow cytometry, the cells were treated with a combination of Retinoic acid, Sonic hedgehog, Brain derived neurotrophic factor, and B27 through a 2-step procedure for two weeks. The expression of MN-specific markers was examined using immunocytochemistry and flow cytometery. Results: Flow cytometery analysis revealed 98% and 95. 7% CD133++ and CD34++ cells, respectively. By the end of the two-week differentiation protocol, CD133++ and CD34++ cells acquired unipolar MNL morphology with thin and long neurites. The expression of Isl-1, AChE, NF-H and Nestin was detected in 66. 4%, 58. 3%, 80. 6%and 84. 9% of CD133++ cells and 63. 2%, 52. 3%, 78. 6% and 80. 1% of CD34++ cells. TConclusion: Human UCB-CD133++ and CD34++HSCs are remarkably potent cell candidates to trans-differentiate into MOTOR NEURON-like cells, in vitro.

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APA: Copy

ALAVI MOGHADDAM, SEPIDEH, FAGHIHI, FAEZEH, & HAYATI ROODBARI, NASIM. (2018). DIFFERENTIATION POTENTIAL OF HUMAN CD133 AND CD34 POSITIVE HEMATOPOIETIC STEM CELLS INTO MOTOR NEURON-LIKE CELLS; AN IN VITRO STUDY. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 28(2 ), 92-103. SID. https://sid.ir/paper/98611/en

Vancouver: Copy

ALAVI MOGHADDAM SEPIDEH, FAGHIHI FAEZEH, HAYATI ROODBARI NASIM. DIFFERENTIATION POTENTIAL OF HUMAN CD133 AND CD34 POSITIVE HEMATOPOIETIC STEM CELLS INTO MOTOR NEURON-LIKE CELLS; AN IN VITRO STUDY. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2018;28(2 ):92-103. Available from: https://sid.ir/paper/98611/en

IEEE: Copy

SEPIDEH ALAVI MOGHADDAM, FAEZEH FAGHIHI, and NASIM HAYATI ROODBARI, “DIFFERENTIATION POTENTIAL OF HUMAN CD133 AND CD34 POSITIVE HEMATOPOIETIC STEM CELLS INTO MOTOR NEURON-LIKE CELLS; AN IN VITRO STUDY,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 28, no. 2 , pp. 92–103, 2018, [Online]. Available: https://sid.ir/paper/98611/en

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