MOLECULAR CHARACTERIZATION OF A 70 KDA HEAT SHOCK PROTEIN (HSP70) GENE IN DERMATOPHYTE PATHOGEN T.VERRUCOSUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOTEVAZE K.; EMAMI M.; FALAHIAN F.A.; KHORAMIZADEH MOHAMMAD REZA; REZAEI SASAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2006 | Volume:16 | Issue:2 Page(s): 57-63

Download Full-Text

Persian Verion

View:

816

Download:

Cites:

Information Journal Paper

Title

MOLECULAR CHARACTERIZATION OF A 70 KDA HEAT SHOCK PROTEIN (HSP70) GENE IN DERMATOPHYTE PATHOGEN T.VERRUCOSUM

Author(s)

Keywords

DERMATOPHYTEQ3

T.VERRUCOSUMQ3

CHAPERONEQ3

HEAT SHOCK PROTEINQ2

Abstract

Background: Trichophyton verrucosum (T.verrucosum) is one of the DERMATOPHYTE fungi which invades the skin of human and animals particularly cattle. Several properties of this fungus have been investigated so far. However a few studies were carried out in the field of molecular biology of this fungus. In the present study we tried to identify the CHAPERONE proteins related to the 70 kDa HEAT SHOCK PROTEINs (HSP70) in this fungus and study of its gene regulation under heat-stress conditions.Materials and methods: Pairs of 21 and 20 nt primers were designed from highly conserved regions of the similar genes in other fungi. Mentioned primers were utilized in PCR by using isolated genomic DNA and cDNA of T.VERRUCOSUM whereas the PCR fragments were then sequenced. Results: Eventually, 2217 nucleotides have been sequenced from this new gene which has two introns and encodes a polypeptide with 654 amino acids. Sequences comparison in gene data banks (NCBI, NIH) for both the complete DNA and its deduced amino acid revealed significant homology with members of the eukaryotic 70 kDa HSP family. Further investigation revealed that the expression of this new gene increases under heat-stress conditions. Conclusion: This new gene has similarity with other genes of the same family in other organisms. This protein could efficiently protect the microorganism against heat stress.

Cites

No record.

References

No record.

Cite

APA: Copy

MOTEVAZE, K., EMAMI, M., FALAHIAN, F.A., KHORAMIZADEH, MOHAMMAD REZA, & REZAEI, SASAN. (2006). MOLECULAR CHARACTERIZATION OF A 70 KDA HEAT SHOCK PROTEIN (HSP70) GENE IN DERMATOPHYTE PATHOGEN T.VERRUCOSUM. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 16(2), 57-63. SID. https://sid.ir/paper/98624/en

Vancouver: Copy

MOTEVAZE K., EMAMI M., FALAHIAN F.A., KHORAMIZADEH MOHAMMAD REZA, REZAEI SASAN. MOLECULAR CHARACTERIZATION OF A 70 KDA HEAT SHOCK PROTEIN (HSP70) GENE IN DERMATOPHYTE PATHOGEN T.VERRUCOSUM. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2006;16(2):57-63. Available from: https://sid.ir/paper/98624/en

IEEE: Copy

K. MOTEVAZE, M. EMAMI, F.A. FALAHIAN, MOHAMMAD REZA KHORAMIZADEH, and SASAN REZAEI, “MOLECULAR CHARACTERIZATION OF A 70 KDA HEAT SHOCK PROTEIN (HSP70) GENE IN DERMATOPHYTE PATHOGEN T.VERRUCOSUM,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 16, no. 2, pp. 57–63, 2006, [Online]. Available: https://sid.ir/paper/98624/en

Related Journal Papers