SALICYLIC ACID INDUCED PROTEINS IN DIANTHUS CARYOPHYLLUS L. SHOOT CULTURE

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NOORI KOOTANAII F.; BERNARD F.; SHAKER H.; FAHIMI H.

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2006 | Volume:16 | Issue:2 Page(s): 65-68

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Title

SALICYLIC ACID INDUCED PROTEINS IN DIANTHUS CARYOPHYLLUS L. SHOOT CULTURE

Author(s)

NOORI KOOTANAII F. | BERNARD F. | SHAKER H. | FAHIMI H. | Issue Writer Certificate

Keywords

TISSUE CULTUREQ4

POLYPEPTIDESQ3

GEL ELECTROPHORESISQ3

SALICYLIC ACIDQ3

CARNATIONQ3

Abstract

Background: The purpose of this study was to achieve defense proteins. Indeed, it proposed that SALICYLIC ACID (SA) involved a number of defense mechanisms. The influence of SA application probably on changes in levels of protein extent of Dianthus shoots was investigated.Methods: Firstly, explants of cv. Cerise Royallette were cultured on OM medium with 0.2 mg.L−1 NAA, 0.5 mg.L−1 BAP, supplemented by 0, 10, 50, and 200 µM SA. After 4 weeks, growth of shoots were measured and then prepared extracts and performed Bradford test. These substances were tested with SDS-PAGE, to find the concentrations which caused changes in quality of proteins.Results: Data analysis indicated that the most typical effect of SA treatment protein content of shoots was occurred. Indeed, treatment increased protein content considerably, however, it decreased the number of shoots. Analysis of the polypeptide pattern following SDS-PAGE showed that all tested SA changed the accumulation of both 38 and 39 kDa POLYPEPTIDES that located very close to each others, and the level of their accumulation was found to be varied depending on the kind and intensity of the stressor.Conclusion: The data provide evidences that plant defense mechanisms might involve the same proteins in response to SA and may lead to induction or deletion of defense proteins changes.

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APA: Copy

NOORI KOOTANAII, F., BERNARD, F., SHAKER, H., & FAHIMI, H.. (2006). SALICYLIC ACID INDUCED PROTEINS IN DIANTHUS CARYOPHYLLUS L. SHOOT CULTURE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 16(2), 65-68. SID. https://sid.ir/paper/98627/en

Vancouver: Copy

NOORI KOOTANAII F., BERNARD F., SHAKER H., FAHIMI H.. SALICYLIC ACID INDUCED PROTEINS IN DIANTHUS CARYOPHYLLUS L. SHOOT CULTURE. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2006;16(2):65-68. Available from: https://sid.ir/paper/98627/en

IEEE: Copy

F. NOORI KOOTANAII, F. BERNARD, H. SHAKER, and H. FAHIMI, “SALICYLIC ACID INDUCED PROTEINS IN DIANTHUS CARYOPHYLLUS L. SHOOT CULTURE,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 16, no. 2, pp. 65–68, 2006, [Online]. Available: https://sid.ir/paper/98627/en

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