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Information Journal Paper

Title

Impact of Laboratory Methods and Gene Targets on Detection of Streptococcus pneumoniae in Isolates and Clinical Specimens

Pages

  216-222

Abstract

 Background: Timely identification of Streptococcus pneumoniae infections can lead to a decrease in mortality rates. Differentiation of S. pneumoniae from other similar species using traditional culture-based and molecular methods is problematic. In this study, we assessed the efficacy of identifying the blpA and lytA for the detection of S. pneumoniae from isolates and various clinical samples using molecular methods. Methods: A total of 440 clinical samples were collected from patients with suspected invasive pneumococcal infections during February 2016 to October 2018. Biochemical tests were used to confirm the dubious colonies on 5% sheep blood agar. Fifty-seven confirmed isolates, 57 culture-positive samples, and 57 culture-negative samples were analyzed for the presence of blpA and lytA using both conventional and real-time PCR. Results: All the isolates and culture-positive samples were positive for blpA and lytA by both PCR methods. Of the 57 culture-negative samples, conventional and real-time PCR amplified blpA from six and two samples, and lytA from seven and two samples, respectively. Conclusions: The specificity of real-time PCR assay was significantly higher than that of conventional PCR for the identification of S. pneumoniae. In addition, it is suggested that respiratory secretions are not suitable specimen for direct diagnosis of pneumococcal infections.

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    APA: Copy

    Mosadegh, Mehrdad, Asadian, Rozita, Emamie, Amir Darb, Rajabpour, Mohammadreza, Najafinasab, Elmira, POURMAND, MOHAMMAD REZA, & AZARSA, MOHAMMAD. (2020). Impact of Laboratory Methods and Gene Targets on Detection of Streptococcus pneumoniae in Isolates and Clinical Specimens. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 9(2), 216-222. SID. https://sid.ir/paper/987274/en

    Vancouver: Copy

    Mosadegh Mehrdad, Asadian Rozita, Emamie Amir Darb, Rajabpour Mohammadreza, Najafinasab Elmira, POURMAND MOHAMMAD REZA, AZARSA MOHAMMAD. Impact of Laboratory Methods and Gene Targets on Detection of Streptococcus pneumoniae in Isolates and Clinical Specimens. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2020;9(2):216-222. Available from: https://sid.ir/paper/987274/en

    IEEE: Copy

    Mehrdad Mosadegh, Rozita Asadian, Amir Darb Emamie, Mohammadreza Rajabpour, Elmira Najafinasab, MOHAMMAD REZA POURMAND, and MOHAMMAD AZARSA, “Impact of Laboratory Methods and Gene Targets on Detection of Streptococcus pneumoniae in Isolates and Clinical Specimens,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 9, no. 2, pp. 216–222, 2020, [Online]. Available: https://sid.ir/paper/987274/en

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