The effect of bromhexine, gentamicin and imipenem on biofilm of standard bacterial Escherichia coli and Pseudomonas aeruginosa by ELISA method

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HAMZEHEE MOHAMAD REZA; NOMANPOUR BIZHAN; AKHAVAN SEPAHI ABBAS

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2019 | Volume:29 | Issue:3 Page(s): 216-221

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Title

The effect of bromhexine, gentamicin and imipenem on biofilm of standard bacterial Escherichia coli and Pseudomonas aeruginosa by ELISA method

Author(s)

HAMZEHEE MOHAMAD REZA | NOMANPOUR BIZHAN | AKHAVAN SEPAHI ABBAS | Issue Writer Certificate

Keywords

BiofilmQ2

Escherichia coliQ2

Pseudomonas aeruginosaQ2

BromhexineQ1

ELISAQ2

Abstract

Background: Biofilms are a collection of microorganisms that have the ability to stick to different levels. Due to the difficulty of treatment of bacterial Biofilm infections and their lack of recognition by conventional diagnostic methods, this study aimed to provide a new method of identification and the effect of related drugs on Pseudomonas aeruginosa and Escherichia coli Biofilms. Materials and methods: In this study, two strains of Escherichia coli and Pseudomonas aeruginosa were used. Using the microplate method, gentamicin and imipenem combined with Bromhexine and also Bromhexine alone with different dilutions were used on the Biofilm of these bacteria. Then results read out using ELISA reader. Results: Bromhexine alone and without combining with other substances or types of antibiotics had a positive effect on preventing the formation of Biofilms and bacterial adhesion, and reduced Pseudomonas aeruginosa and Escherichia coli in the Biofilm. Conclusion: Biofilm has been able to grow significantly, despite the use of strong antibiotics such as imipenem and gentamicin. Its existence in the body, or devices such as urethral catheters, cardiac artificial valves or other artificial organs, causes a significant disorder within immune system.

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APA: Copy

HAMZEHEE, MOHAMAD REZA, NOMANPOUR, BIZHAN, & AKHAVAN SEPAHI, ABBAS. (2019). The effect of bromhexine, gentamicin and imipenem on biofilm of standard bacterial Escherichia coli and Pseudomonas aeruginosa by ELISA method. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 29(3 ), 216-221. SID. https://sid.ir/paper/98865/en

Vancouver: Copy

HAMZEHEE MOHAMAD REZA, NOMANPOUR BIZHAN, AKHAVAN SEPAHI ABBAS. The effect of bromhexine, gentamicin and imipenem on biofilm of standard bacterial Escherichia coli and Pseudomonas aeruginosa by ELISA method. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2019;29(3 ):216-221. Available from: https://sid.ir/paper/98865/en

IEEE: Copy

MOHAMAD REZA HAMZEHEE, BIZHAN NOMANPOUR, and ABBAS AKHAVAN SEPAHI, “The effect of bromhexine, gentamicin and imipenem on biofilm of standard bacterial Escherichia coli and Pseudomonas aeruginosa by ELISA method,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 29, no. 3 , pp. 216–221, 2019, [Online]. Available: https://sid.ir/paper/98865/en

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