Antiproliferative and apoptotic activity of gemcitabine-lauric acid conjugate on human bladder cancer cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Wang Hongxia; Shao Zhiyu; Xu Zhiwen; Ye Binghao; Li Ming; Zheng Qiaoqiao; Ma Xingyuan; Shi Ping

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2022 | Volume:25 | Issue:4 Page(s): 536-542

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Antiproliferative and apoptotic activity of gemcitabine-lauric acid conjugate on human bladder cancer cells

Author(s)

Keywords

Apoptosis

Cell cycle checkpoints

Gemcitabine

SLC29A1 protein

Urinary bladder neoplasms

Abstract

Objective(s): Gemcitabine is a first-line drug for the treatment of bladder cancer. One of the most important mechanisms of Gemcitabine resistance is the low expression of cellular membrane transporter hENT1. Various derivatives containing fatty acid side chains have been developed in order to facilitate Gemcitabine uptake and prolong its retention in cells, such as CP-4126. In this study, the anti-tumor effect and mechanism of a new derivative of Gemcitabine named SZY-200 on bladder cancer cells were investigated. SZY-200 was assembled from the Gemcitabine-lauric acid conjugate. Materials and Methods: Antiproliferative activities of SZY-200 and lauric acid were evaluated using CCK-8 assay and clonogenic survival assay. The hENT1 inhibitor NBMPR was employed to determine the role of hENT1 in the apoptotic activity of GEM, CP-4126, and SZY-200. RT-qPCR, flow cytometry, fluorescence microscope, western blotting, and wound healing assay were used to study the mechanisms of SZY-200. The target genes were predicted using the BATMAN-TCM database. Results: Our data showed that SZY-200 could inhibit the proliferation of bladder cancer cells by inducing cell cycle arrest and Apoptosis. The inhibitory effects were comparable to Gemcitabine and CP-4126. SZY-200 does not rely on hENT1 to help it enter bladder cancer cells. Also, we found that lauric acid could inhibit the proliferation of bladder cancer cells. SZY-200 could down-regulate the expressions of PPARG and PTGS2 which were related to the occurrence and development of bladder cancer. Conclusion: SZY-200 has the same or more advantages as CP-4126 and could be an ideal candidate drug for further in vivo investigation.

Cites

No record.

References

No record.

Cite

APA: Copy

Wang, Hongxia, Shao, Zhiyu, Xu, Zhiwen, Ye, Binghao, Li, Ming, Zheng, Qiaoqiao, Ma, Xingyuan, & Shi, Ping. (2022). Antiproliferative and apoptotic activity of gemcitabine-lauric acid conjugate on human bladder cancer cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 25(4), 536-542. SID. https://sid.ir/paper/991175/en

Vancouver: Copy

Wang Hongxia, Shao Zhiyu, Xu Zhiwen, Ye Binghao, Li Ming, Zheng Qiaoqiao, Ma Xingyuan, Shi Ping. Antiproliferative and apoptotic activity of gemcitabine-lauric acid conjugate on human bladder cancer cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2022;25(4):536-542. Available from: https://sid.ir/paper/991175/en

IEEE: Copy

Hongxia Wang, Zhiyu Shao, Zhiwen Xu, Binghao Ye, Ming Li, Qiaoqiao Zheng, Xingyuan Ma, and Ping Shi, “Antiproliferative and apoptotic activity of gemcitabine-lauric acid conjugate on human bladder cancer cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 25, no. 4, pp. 536–542, 2022, [Online]. Available: https://sid.ir/paper/991175/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops