Recombinant PBP2a/autolysin conjugate as PLGA-based nanovaccine induced humoral responses with opsonophagocytosis activity, and protection versus methicillin-resistant Staphylococcus aureus infection

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HAGHIGHAT SETAREH; SIADAT SEYED DAVAR; AKHAVAN SEPAHI ABBAS; MAHDAVI MEHDI

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2022 | Volume:25 | Issue:4 Page(s): 442-450

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Title

Recombinant PBP2a/autolysin conjugate as PLGA-based nanovaccine induced humoral responses with opsonophagocytosis activity, and protection versus methicillin-resistant Staphylococcus aureus infection

Author(s)

HAGHIGHAT SETAREH | SIADAT SEYED DAVAR | AKHAVAN SEPAHI ABBAS | MAHDAVI MEHDI | Issue Writer Certificate

Keywords

Autolysin

Methicillin-resistant-Staphylococcus aureus

Nanovaccine

PBP2a

Abstract

Objective(s): Methicillin-resistant Staphylococcus aureus (MRSA) reasons extreme infections, can resist various conventional antimicrobial agents, and cause morbidity and mortality worldwide. Vaccination seems to help modulate MRSA infections. Nanovaccine is considered a novel strategy in vaccine technology. The primary purpose of the present study was to develop a conjugate vaccine based on recombinant PBP2a and MRSA Autolysin formulated in PLGA as a nanoparticle capable of enhancing protective responses against MRSA in the murine model. Materials and Methods: Recombinant PBP2a and Autolysin have been expressed and purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity column and characterized by SDS-PAGE and western blot. PLGA was bound to recombinant proteins by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) and adipic acid dihydrazide (ADH) as a linker and spacer, respectively. Conjugation of recombinant proteins to PLGA was confirmed by the AFM assay, zeta potential, and size distribution, and its efficacy was evaluated in mice. Total IgG, IgG1, IgG2a, IgG2b, and IgM titers were analyzed to assess immune responses. Lastly, the bioactivity of antibodies was tested by using the opsonophagocytosis assay. Results: Mice immunized with the r-PBP2a-r-Autolysin– PLGA Nanovaccine led to increased levels of opsonic antibodies and IgG1, IgG2a, IgG2b, and IgM when compared with other experimental groups. Our results confirmed that vaccination with Nanovaccine could reduce the mortality rate against the sub-lethal dose of MRSA challenge. Furthermore, the Nanovaccine could eliminate MRSA from the kidney of infected mice. Conclusion: This study may provide valuable insights into the protective power of the r-PBP2a-r-Autolysin– PLGA conjugate vaccine against MRSA infection.

Cites

Investigating the Effect of Monophosphoryl Lipid A as a Co-adjuvant on Humoral Immune Response against Staphylococcus Aureus Infection in a Mouse Model

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APA: Copy

HAGHIGHAT, SETAREH, SIADAT, SEYED DAVAR, AKHAVAN SEPAHI, ABBAS, & MAHDAVI, MEHDI. (2022). Recombinant PBP2a/autolysin conjugate as PLGA-based nanovaccine induced humoral responses with opsonophagocytosis activity, and protection versus methicillin-resistant Staphylococcus aureus infection. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 25(4), 442-450. SID. https://sid.ir/paper/991479/en

Vancouver: Copy

HAGHIGHAT SETAREH, SIADAT SEYED DAVAR, AKHAVAN SEPAHI ABBAS, MAHDAVI MEHDI. Recombinant PBP2a/autolysin conjugate as PLGA-based nanovaccine induced humoral responses with opsonophagocytosis activity, and protection versus methicillin-resistant Staphylococcus aureus infection. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2022;25(4):442-450. Available from: https://sid.ir/paper/991479/en

IEEE: Copy

SETAREH HAGHIGHAT, SEYED DAVAR SIADAT, ABBAS AKHAVAN SEPAHI, and MEHDI MAHDAVI, “Recombinant PBP2a/autolysin conjugate as PLGA-based nanovaccine induced humoral responses with opsonophagocytosis activity, and protection versus methicillin-resistant Staphylococcus aureus infection,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 25, no. 4, pp. 442–450, 2022, [Online]. Available: https://sid.ir/paper/991479/en

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