Due to the increasing use of herbal items in aquatic ration, the amount of anti-nutritional substances in aquatic ration has increased. One of the ways to reduce the effects of anti-nutritional substances of non-starch polysaccharides is using enzyme supplements in aquatic feed.In the present study, at first, the minimum required amount of alpha-amylase, cellulase, xylanase and beta-glucanase enzymes for wheat and soybean flour was obtained by Miller's sugar test method.Carp fish with an average initial weight of 10.77±1.75 in 5 treatments and 3 repetitions including ration without enzyme (control treatment), ration containing enzyme which was added to the food as a spray after manufacturing (treatment 1), ration containing enzyme which added to its substrate 6 hours after production (Treatment 2), ration containing enzyme added to substrate 12 hours before production (Treatment 3) and ration containing enzyme added to substrate 24 hours before production (Treatment (4), was fed with ration containing multi-enzyme for 60 days.The results of the experiment showed that the total weight in treatment 3 (23.09±1.5) compared to the control treatment (17.67±0.48) increased significantly (P≤0.05).Also, the investigations of this experiment showed that the use of enzymes improves the growth indices and food conversion ratio of common carp (P≤0.05).In this study, the combination of enzymes in treatment 3 increased the apparent digestion of protein and fat compared to the control (P≤0.05).