Objective: Adolescence, a period of instability and significant changes, sees engagement in health behaviors as pivotal for enhanced health and longevity. Consequently, this study aims to investigate the connection between health literacy and health behaviors, striving to gauge the multifaceted impact of health literacy and educational aspects on health behaviors. Method: This cross-sectional, descriptive-analytical study was conducted in 1402 and focused on adolescents aged 12 to 20 in the Semnan province. The sample consisted of 382 adolescents from Semnan and Mehdi City. Utilizing Kanbari's Health Literacy assessment and Walker's Health-Promoting Behaviors Inventory (HPLP), data were collected and analyzed using Pearson and simple linear regression, independent t-test, and SPSS-24 software. Results: Independent t-test analysis for gender groups showed a significant difference in health behaviors between boys and girls (t351 =-2. 14, P = 0. 033 two-tailed). Components like interpersonal support (t345 =-2. 488, P = 0. 013 two-tailed) and exercise (t341 =-4. 68, P = 0. 001 two-tailed) also demonstrated substantial disparities. Boys scored higher on all dimensions of health behavior compared to girls and exhibited significantly greater self-efficacy in the health literacy domain (t380 =-2. 63, P = 0. 009 two-tailed). The relationship between health literacy components and health behaviors displayed the highest predictability for health behaviors (r = 0. 417, P = 0. 001). A moderate positive correlation emerged between health literacy and health behaviors (p < 0. 001, r = 0. 583), with health literacy accounting for 34% of health behaviors (d = 0. 51, p = 0. 001, R2 = 0. 34). Conclusion: Elevating health literacy through education can play a significant role in enhancing health behaviors among adolescents, laying a strong foundation for future community health.