Background: Considering the high prevalence of zinc deficiency in Iran and important role of zinc in health of men, especially diabetic patients, this study was carried out to determine the effects of zinc supplementation on zinc, insulin and glucose levels in men with type 2 diabetes mellitus aged 50-65 years.Materials & Methods: A randomized double-blind placebo controlled clinical trial was conducted on 54 diabetic patients. None of them suffered from any disease other than type 2 diabetes mellitus. The subjects were matched according to age, duration of the disease, body mass index (BMI) and waist to hip ratio (WHR).Then subjects were randomly assigned to receive either a zinc supplement (25 mg Zn/day, in sulfate form) or placebo for 2 months. Before and after the study period, fasting blood samples was taken from each patient to determine blood glucose, HbA1c, insulin, C-peptide concentrations, serum and leukocytes zinc. 2hpp and urinary glucose and zinc concentration were measured.Results: After treatment mean fasting blood glucose and 2hpp increased in both groups (p£0.01). Also urinary glucose concentration increased, but it was not significant. Mean HbA1c, serum insulin and C-peptide decreased significantly in test and control groups (p<0.01). After treatment serum zinc decreased significantly in both groups, while leukocytes zinc increased (p<0.01). In this study, no significant difference was seen between two groups.Conclusion: In the present study, zinc supplementation had no significant effects on zinc, insulin and glucose levels in diabetic subjects.