Annals of Military and Health Sciences Research
Year:2012 | Volume:10 | Issue:3 (SERIAL NUMBER 39)|Papers Count:12

Background: Previous studies revealed that Vanadium complexes exhibit interesting biological properties. The goal of this study was to examine the antiproliferative and apoptosis induction virtue of some newly synthesized Schiff-base of vanadium (VOL Complex: C17H16N2O3V and C19H20N2O5V) on K562 leukemia cells.Materials and Methods: In this experimental study, the leukemia cells were cultured in RPMI medium in 25 and 400 mg/L concentration and they were incubated for 48 hours. The vital activity of cells was assessed by MTT method. Apoptosis induction percentage was measured in 12, 24 and 48 hours after encountering with 150, 250 and 350 mg/L concentrations of VOL Complex by Annexin-PI kit and flow cytometry Cell cycle analysis was performed in the 150 mg/L concentration at 24 hours with flow cytometry.Results: The MTT results showed that the K562 cells viability were sensitive to VOL complex in a concentration. dependent manner With increase in Schiff-base of vanadium concentration the survival percentages of K562 leukemia cells were decreased to 90, 85 and 80 percent. Encountering cells with VLO complex caused to induce apoptosis in relation with time and concentration. The highest apoptosis (34.11 percent) was happened in 350 mg/ml at 48 hours.Conclusion: This finding indicated that VOL complex, even in noncytotoxic dose, have just an apoptotic inducing effect and capable of arresting the affected cell in Go/G1 phase of cell cycle.

Background: Today, the consumers have more knowledge about the unwanted effects of synthetic antimicrobials in foods and they want food with more natural ingredients. There are many researches about antimicrobial effects of essential oils, but we did not found researches about Zatariamultiflora Bios on E.coli O157: H7 in minced beef. The aim of this study was to determinre the effect of Zatariamultiflora Bios essential oil on E.coli O157: H7 in minced.Materials and Methods: The antimicrobial effect of different concentrations of Zatariamultiflora Boiss essential oil at supplementation levels of (0, 0.005, 0.015, 0.03%) on E. coli O157: H7 was examined in minced beef. All of the above concentrations showed acceptable organoleptic properties in minced beef. Eo at 0.03% possessed a strong antibacterial activity against E.coli O157: H7 in minced beef.Results: The correlation coefficient of different concentrations of ZatariamultifloraBoiss essential oil with logarithm of the numbers of E. coli O157: H7 was –0.701, –0.599 at 4 and 10°C respectively. It was found that effect of different concentrations of essential oil on growth rate of E. coli O157: H7 was statistically significant (p<0.01).Conclusion: In this study, it was found that treatment of minced beef with different concentrations of essential oils showed an inhibitory activity against E. coli O157: H7 during storage at 4oC, but not at 10oC (p<0.01). According to present study, storage at a proper refrigerated storage like 4oC is suggested. ZatariamultifloraBioss essential oil can use as a natural preservative instead of chemical preservatives for meat products.

Background: Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damages. Usually free radicals are scavenged and detoxified by antioxidants. In this study we assessed antioxidative role of NGAL (Neutrophil gelatinase associated lipocalin=Lipocalin2) molecule on crushed sciatic nerves of rats.Materials and Methods: In this study 40 rat's sciatic nerves were crushed and then at day 1 and 3 and week 1, 3, 5 after injury the sciatic nerve of rats which suffering crushed were extracted. First of all total mRNA of samples was extracted and then cDNA was synthesized. The expression of NGAL gene was confirmed by using semiquantitative RT-PCR analysis. For immunohistochemistry analysis, the samples were fixed in praformaldehyde and cut in 20 micrometer slices by cryostat.Results: The results showed that the expression of lipocalin 2 was significantly higher in day 1, day 3 and week 1 in crushed sciatic nerves in compare with the intact (non-injured) nerves (p<0.0001). Immunohistochemistry results also confirmed the protein expression of these genes.Conclusion: The sciatic nerve injuries cause oxidative stress which followed by crush injury. In this research NGAL molecule showed maximum up-regulation in degeneration process after injury when oxidative stress have maximum peak. In this project for the first time we demonstrated the role of this molecule in degeneration and regeneration process of peripheral nerves and with more studies it may suggest as new complemental treatment.

Background: Candida albicans is an important human pathogen that can causes systemic infections, predominantly among populations with weak immune systems. Candida spp. produces biofilms on synthetic materials such as catheter, which facilitates adhesion of the organisms to devices. Since the frequent replacement of these devices is difficult and expensive to achieve solutions for rapid identification and control of biofilm infections is essential. The purpose of this study is, the use of zinc oxide nanoparticles in the manufacture catheter and evaluation of its inhibitory effect in preventing of the biofilm formation.Materials and Methods: In this experimental study, the catheters in making poly vinyl chloride, Znonano particles with aconcentration 2.027mg/ml was used. Biofilms were formed over a series of time intervals (2, 4, 6, 8, 24 and 48 h). At each time interval, biofilm formation was measured with the XTT and biomass assay and concurrently assessed by light microscopy.Results: Results showed that catheters containing zinc oxide increases with time in cell density (absorbance increase) were lower than those without catheters. Similar results were also measured in dry weight.Conclusion: This study demonstrated antifungal activity of ZnO. therfor biofilm formation on medical equipment such as catheters, implants, dentures, artificial heart valves, etc can be inhibited by ZnO nanoparticles.

Background: Quality of Life is people perception of their position in life regard to the cultural context and values which they live in. It is in relation with their goals, expectations, standards and concerns. In recent years increasing in quality of life of hypertensive patients became a goal in national health systems. The aim of this study was to compare health related- quality of life in male military personnel with and without hypertension.Materials and Methods: In this descriptive-comparative study, 200 male military (100 hypertensive patients and 100 healthy men) was selected with convenience sampling in Tehran. The quality of life was assessed by short form (SF36) quality of life questionnaire. The data was analyzed by independent t- test and chi-square.Results: Mean age in hypertension group was 8.48±44.83 and in the compare group was 50±40.44. The quality of life was 82.20±10.72 and 72.77±17.60 in hypertensive and compare group. The quality of life was higher in the compare group (P<0.001).Conclusion: Quality of life in chronic diseases such as hypertension is lower. Therefore there is need for more attention on early detection and better treatments based on the quality of life in people with hypertension.

Background: Fear of labor pain is one of the problems that raise rate of caesarean section in Iran. Midwives can use one of the nonpharmacological pain relief methods such as reflexology and encourage mothers to use normal vaginal delivery. The aim of this study was to determine the effect of reflexology on anxiety during labor in primiparous.Materials and Methods: This clinical trial (IRCT: 0022) was carried out on 80 primiparous women with low risk pregnancy that referring to Karaj hospitals. Patients were randomly assigned in two groups. Intervention group received reflexology for 40 minutes. Level of anxity before and after intervention was measured with Spielberger's State-Trait Anxiety Questionnaire. The data was analyzed by t-test and Repeated measure ANOVA tests.Results: There was no significant differences between two groups before the intervention (p=0.15). After intervention the difference in anxiety score was significant between two groups (p<0.001).Conclusion: Reflexology can lead to decrease anxiety during of labor. Therefore, this noninvasive technique can be used for decrease of anxiety and encourage mothers to use normal vaginal delivery.

Background: Type II diabetes is the most common metabolic disease in the world. The aim of present study wos to determine the effects of whortleberry on glucose control index and lipid profile in patients with type II diabetes.Materials and Methods: The present study was a randomized trial (IRCT: 1389). The sample was 75 patients with type II diabetes (30 people in intervention and 45 people in control group) in Karaj-Iran. The control groups took placebo capsules (starch) and whortleberry (500 mg, twice a day after breakfast and lunch) was administered in intervention group. Vein blood samples were taken at the beginning and end of study to measure fasting blood glucose, glycosylated hemoglobin (HbA1c), insulin serum, Triglyceride, total Cholesterol and its contents. Using HOMA Score, insulin resistance was also measured. Descriptive statistics and chi-squared, T- independent, paired T and ANCOVA testes were used for data analysis.Results: After 90 days of the intervention, there was a significant difference in FBS, two hour blood sugar (BS2hpp) and HOMA Score between Whortleberry and control group. Also In whortleberry group, in all glucose control indexes, there were significant differences After 90 days of the intervention.Conclusion: This study demonstrated that using of oral whortleberry supplement for adjusting blood glucose is suitable for patients with type II diabetes patients. Therefore using of it along with medical treatment can be recommended.

Background: Non-varicose upper gastrointestinal bleeding is one of the common causes of hospitalization with high mortality and morbidity. The aim of this study was to investigate the effect of Octreotide in treating non-varicose upper gastrointestinal bleeding.Materials and Methods: In this clinical trial with IRCT: 1361, patients with gastrointestinal bleeding, with neither gastrointestinal varices nor hepatic failure, were enrolled into two groups designated respectively as A and B. Patients in group A received routine treatment. In group B, in addition to routine treatment, patients received 50 mg bolus Octreotide and 25 mg/h infusions. For comparison between the two groups the difference hemoglobin at arrival time, hemoglobin the time of discharge, length of stay in the emergency department, required blood transfusion, the recurrence rate of bleeding and death rate were assessed.Results: In this study, 80 patients in both groups were examined. There was no difference in age and sex between two groups. Hemoglobin on arrival in the two groups showed no significant difference, but hemoglobin level was different at the discharge time. Our study showed no significant difference between two groups about average of received pack Cell. Our results demonstrated that length of stay at emergency department, decreased hemoglobin and bleeding recurrence rate had no significantly difference between two groups.Conclusion: The result showed that Octreotide can't add an extra value for treating upper varicosdee gastrointestinal bleeding.

Sampling in qualitative and quantitative research are very different, because it aims, gaining a deep understanding of the phenomenon is being investigated, rather than generalizing the results. Participants Selection in the qualitative study is done with aim of achieve the maximum information about the phenomenon under study. In quantitative research there is too much emphasis on a random selection, an equal chance for selection of all members of the research community, but in the qualitative study samples or participants are selected or invited. Purposive sampling that also is called purposeful or qualitative sampling that means purposeful selection of samples for acquiring knowledge or information. This type of sampling does not focus on developing the fixed and immutable rules or generalizing the results but try to better understand the phenomenon in special field. Purposive sampling consists of three main types including "Sampling to Achieve Representativeness or Comparability", "Sampling Special or Unique Cases" and "Sequential Sampling". Saturation in qualitative research as a method of sampling is considered the gold standard. In this paper, we tried to introduce the participant's selection methods and the differences between quantitative and qualitative method and the strategy for decision making about data saturation will be offered.

Background: Optimal performance in spatial explorations depends on appropriate nutrition. Sufficient nutrition is the basis of human physiologic activities. Zero gravity leads to physiological changes in astronaut's body. If the astronaut has no healthy situation, spatial mission will defeat.Material & Methods: The article is a collection of data & knowledge regarding to earth atmosphere from credible & scientific books & articles.Results: Obtaining of macro & micro nutrition for astronauts is very important & if their foods (because of anorexia) were not consumed, it has the adverse consequences. Many of reasons detected in association with astronaut's anorexia. Various researches show that no rapid stool defecation duo to decrease of energy reception. Thus, accurate regulation of energy reception & consumption should be necessary.Conclusion: Research results showed that total energy consumption in the earth & space are equal but ratio of materials that generate this energy are variable & need to be calculation. This article presented in aerospace nutrition conference.

Background: Eosinophilic granuloma is a rare disease that more occurs in children especially bays. It usually presents in flat and long bones. Sometimes skull and vertebral spine is affected.Case Report: The Case was a 6 years old boy that had back pain from 20 days ago. In physical exam we found a local tenderness on tenth thoracic vertebra. Imaging study includes X-ray and MRI revealed vertebra plana on tenth thoracic vertebra without any periostic reaction or bone formation. Bone scintigraphy showed increased radiotracer uptake only in the tenth thoracic vertebra. With attention to age, gender, clinical features, lab data and imaging study we suspected an eosinophilic granuloma for this patient that histological examination confirmed it. With considering to patient’s signs, conservative treatment include thoracolumbosacral brace and long term follow up was used.Conclusion: One of the differentional diagnosis of backpain in children specially in boys is Eosinophilic granuloma.