This study aimed to compare brain-behavioral systems and positive and negative affects in patients with migraine and healthy individuals. One hundred and twenty males and females suffering from migraine headache were selected from medical centers of Dezful and Andimeshk cities using available sampling. The comparison group consisted of 120 healthy males and females who were matched for age, sex and education. Both groups completed the Gray-Wilson Personality Questionnaire (Wilson, Barrett, &Gray, 1989) and the Positive and Negative Affect Scale (Watson, Clark, & Tellegen, 1988). The results of multivariate variance indicated that patient and healthy individuals had significant differences in behavioral inhibition systems, passive avoidance, flight, and negative affect. There were no significant differences between groups in the other approach system variables, including active avoidance‚ extinction‚ fight‚ and positive affect.