In order to study the quantitative and qualitative characteristics of sunflower (Alestar hybrid) under the effects of bio fertilizer and winter wheat as a green manure in combination with the integrated nitrogen sources (chemical-farmyard manure, FYM), an experiment was carried out on experimental farm at faculty of agriculture, Tarbiat Modares University, Tehran, Iran in 2008. The experimental design was a split plot in a randomized complete blocks with three replications, in which seven fertilizer treatments including F1 (100% FYM), F2 (75% FYM+25% chemical), F3 (50% FYM+50% chemical), F4 (25% FYM+75% chemical), F5 (100% chemical), F6 (50% FYM+50% chemical+green manure), F7 (75% FYM+25% chemical + green manure) were randomized to the main plot units and two levels of bio fertilizer I1 (inoculation) and I0 (control) to the sub plot units. The results revealed that grain and biological yield, yield components in integrated systems was significantly more than organic and chemical systems. The highest grain yield (3034.5 kg/ha), head weight (90.06 g), head diameter (19.40 cm), 1000 seed weight (67.99 g) and seed number in head (925.5) were obtained in F6 treatment (F6>F3>F4>F7>F2>F5>F1). Inoculation of bio fertilizer improved the grain yield and yield components, oil and protein content and the oil quality of sunflower compared with untreated plants. Saturated fatty acids decreased significantly, while unsaturated fatty acids (linoleic acid and oleic acid) increased in response to raising the organic manure and using bio fertilizer.