Introduction: Hospital managers and healthcare providers should access to hospital information system (HIS) in order to enhance their efficiency and effectiveness in the organization. Accurate, appropriate, precise, timely and valid information as well as an appropriateness information system are required for their tasks and a basis for decision-making in various levels of the hospital management. The present study was conducted to assess selected HIS in hospitals affiliated to Isfahan University of Medical Sciences, Iran, according to ISO 9241-10.Methods: This was an applied, descriptive cross-sectional study in which seven selected HIS medical records modules were assessed using ISO 9241-10 questionnaire consisting 74 ranked components and entities in hospitals of Isfahan University of Medical Sciences. The content validity and reliability were confirmed and the obtained data were analyzed through SPSS software, and descriptive statistics were used to examine measures of central tendencies.Results: Data analysis suggested the following percentages concerning the software: 68% appropriateness for user’s tasks, 67% self-descriptiveness, 70% controllability by user, 74% compatibility with user’s expectations, 69% error tolerance, 53% possibility of individualization, and 68% possibility of desired learning for user. The total acceptability with the ISO 9241-10 Software was 67%.Conclusion: Information is the basis for policy and decision-making in various levels of the hospital management. Consequently, it seems that developers of HIS should reduce HIS errors and increase its appropriateness for the tasks, self-descriptiveness, controllability, conformity with user’s expectations, error tolerance, appropriateness for individualization and appropriateness for user’s learning.