QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL
Year:2008 | Volume:1 | Issue:4|Papers Count:10

Background and objective: Complications in diabetes mellitus are associated with free radicals and oxidative stress. The human body prevents these complications through antioxidant defense mechanisms. The aim of the study was to investigate the effect of aqueous seed extract of Securigera Securidaca on erythrocyte catalase activity in type1 diabetic rats. Methods: At the present interventional study thirty male wistar rats were used. Animals were divided to two groups including normal and diabetic (n=15 per each group).Each group was divided further to control and experimental subgroups. The experimental subgroups were received 100 and 200 mg/kg/day of the plant extract intraperitoneally. After thirty days administration, blood sample was directly collected from the heart and erythrocyte catalase activity was assessed.Results: catalase activity decreased in diabetic control group significantly (P=0.002).Furthermore, catalase activity in groups treated at two doses of 100mg/kg and 200mg/kg was significantly different as compared to control group (P=0.003). Conclusion: The aqueous seed extract of Securigera Securidaca probably could be effective in decreasing diabetic complications through improvement of antioxidant response by altering catalase activity and consequently reducing oxidative stress.

Background and objective: Cannabinoids which are active compounds of marijuana show some pharmacological effects similar to the opioids. There are also functional interactions between both cannabinoid and opioid systems. In this study we investigated the role of cannabinoid receptors in central amygdala and its interaction with opioid system.Methods: In the present study, we investigated the effects of intraperitoneal injection of opioid drugs on response-induced by intra-amygdala (intra-Amyg) microinjection of cannabinoid agents in rats, using elevated plus-maze test of anxiety.Results: Intraperitoneal injection of morphine (3, 6 and 9 mg/kg) increased %OAT and %OAE, but not locomotor activity, showing an anxiolytic response. However, some doses of the opioid receptor antagonist, naloxone reduced %OAT and locomotor activity as well. Intra-Amyg administration of CB1 cannabinoid receptor agonist, ACPA (at the dose of 1.25 and 5 ng/rat) increased %OAT and %OAE but not locomotor activity, thus showing an anxiolytic response, which was increased by morphine (6 mg/kg, i.p.) without any interaction. Naloxone also reduced ACPA effects.Intra-Amyg administration of CB1 cannabinoid receptor antagonist, AM251 (2.5, 25 and 100ng/rat) did not alter %OAT and %OAE but higher doses of drug (25 and 100 ng/rat) reduced locomotor activity. However, the drug in combination of morphine anxiolytic response and with naloxone decreased anxiety. Conclusion: The results may indicate an anxiolytic for CB1 cannabinoid. Our results also showed that opioid system may have interaction with cannabinoid receptor in the amygdale.

Background and Objective: Non-traumatic acute abdominal pain is one of the most common complaints in patients referred to emergency department. To make diagnosis decision several diagnostic tests such as WBC count, urine analysis, and abdominal sonography are usually ordered which disturb emergency department, staff and force significant cost to patient and are time consuming.This study was done for determining the tests frequency for diagnosis of patients presenting in ED of Hazrat e rasoul hospital with non traumatic acute abdominal pain that had been dismissed from hospital in comparison to previous studies to find whether we can omit some unnecessary tests.Methods: Study method was descriptive-cross sectional. By sequential sampling, the data of 400 dismissed patients with non-traumatic acute abdominal pain were gathered and interpreted by statistical descriptive methods.Results: Participants were 29-22 years old, 40.8% male and 59.2% female. WBC had been ordered in 92% of cases. Urine analysis and abdominal sonography had been ordered 91.7% and 95.5% respectively. Frequency of CBC ordering was similar to previous studies while was 2 and 4 fold more for UA and abdominal sonography in comparison to previous studies.Conclusion: These results necessitate revising the diagnostic tests for non-traumatic acute abdominal pain to omit unnecessary tests.

Background and objective: Hydrogen peroxide solution with stabilized formulation with oligodynamic amounts of silver ion (trade mark: Nanosil) recently became a popular disinfectant for fruits, vegetables, surfaces, medical instruments and so on.Methods: This study was based on determining efficiency of hydrogen peroxide-silver ion complex in six different concentrations measured through removal of total coli forms from samples taken from Qom wastewater. All six different concentrations of disinfectant were incubated separately with wastewater for one hour. Sample size was 12 and sampling was done weekly. Results: In the concentration of 80 and 480 mg/l, logarithmic removal value of total coli forms was 1.9 and 4.5 respectively. In CT value 8600(mg/l).min and more, the total coli forms effluent standard for surface water discharge and agriculture irrigation was achieved (Iranian environmental protection agency act).Conclusion: Use of hydrogen peroxide-silver ion complex as a water and wastewater disinfectant, has some benefits such as elimination of hazardous by products, measurable residual amount and so on. However its application in wastewater effluent is more costly than other usual disinfectants so it is not economically advisable.

Background and objective: Intraoperative bleeding, which reduces visibility in the operative field, is one of the major problems of head and neck surgeries. Improvement of intraoperative visibility and reduction of bleeding is an important task for an anesthetist during head and neck surgery. It has been shown that preoperative beta-blockade decreases bleeding during the operation. Methods: In a 18 month period, 88 patients , who were candidate for nasal procedures in hazrat Rasul medical complex, were selected in a randomized open clinical trial study. They were divided to 4 groupes:50 mg metoprolol at night before surgery 50 mg metoprolol in the morning of the surgery 50 mg metoprolol at night before surgery and in the morning of the surgery placeboHeart rate, Systolic and diastolic blood pressure was measured in a non-invasive way just both upon arrival on the operation bed and after induction of anesthesia during operation. For evaluation of the visibility of the operative field during operation the quality scale proposed by Fromm and Boezzart was used.Results: SPSS13 was used for assessment. There was significant relationship between metoprolol administration and bleeding during the operation. All patients who received two doses of metoprolol one in the last night and another in the morning of the operation day had only mild bleeding. There was statistically significant relation between agitation and time of administration of metoprolol.Conclusion: Nowadays, there are growing evidences that not only decrease in systolic blood pressure but also a low heart rate (< 60 beats per minute) can minimize surgical bleeding. Both of the above mentioned effects could be induced by beta blockers. In this study, two doses of metoprolol could decrease the amount of bleeding and improve the operation field significantly and also could decrease the agitation in recovery room.

Background and objective: Matrix Metaloproteinases (MMPs), produced mainly by fibroblasts, play a major role in wound healing processes. This study aimed to investigate the MMP2 activity in dermal fibroblasts found in chronic contact nickel dermatitis lesions.Methods: In order to study the role of MMP2 in contact nickel dermatitis, fibroblast from patients and healthy individuals were cultured based on ex-plantation of skin. MMP2 activity was measured in fibroblast culture media using zymoanalysis. Zymograms were then analyzed by quantitative densitometry. MTT assay was also used to evaluate and compare proliferation capacity of fibroblasts in both cell cultures.Results: The mean MMP2 activity 6-8 days after ex-plantation was significantly higher in patients fibroblasts than in normal individuals (170±9.2 and 134.3±5.9 in patients and normals, respectively (p<0.05)). The proliferation capacity of patients' fibroblasts was significantly higher than that of normal cases (385938±2816 and 270261±6527 respectively, p<0.05) in the same days.Conclusion: Our study shows a significant increase in degenerative activity of fibroblasts in nickel dermatitis lesions. It was also seen that the MMP2 activity per cell was significantly higher in patients' fibroblasts compared to healthy individuals and that gelatinolytic activity of MMP2 is independent of cell number. Therefore, intra- and inter-cellular signals may be altered in fibroblasts of nickel dermatitis lesions which lead to promotion of fibroblast response to mitogenic and fibrogenic stimulations.

Background and objectiveNoise threats health of many groups of industrial workers and causes hearing loss. Use of personal protective device is the best control method to protect against hazardous conditions. Hence, this investigation was carried out to determine situation of using of protective devices and effective parameters on it, in Qom province workers community in 2006.MethodsThis research is descriptive-sectional study. Sample volume was designed 378 persons working in factories in Qom. First of all, list of Qom factories with noise pollution problems, were collected and 30 important factories among them were selected randomly. In the second stage, 378 persons were selected randomly from workers. The interest information was obtained by questionnaire and collected data were analyzed by SPSS software.ResultsThe obtained results showed that, 83.6% of workers have been using ear protective devices. 296 of them, which were using ear protective devices, had an occupational hygienist in their workplaces. This research also showed that, 109 workers that used ear protective devices, had moderate knowledge level. Moreover, 82.5% of trained workers have used ear protective devices. The statistical analysis of the results showed that there were no significant relationship between use of ear protective devices and existence of occupational hygienist in workplace, knowledge and age of workers, worker’s antecedent, physical health of workers and kind of ear protective devices (p> 0.05).ConclusionThese results showed that among all considered parameters; only four parameters were effective in using ear protective devices; education of workers before employment, head workman and employer’s knowledge level, factories facilitation and kind of ear protective devices.

Background and objectiveOvarian cancer is one of the common gynecological cancers. Meanwhile it is the most lethal cancer and cause of more than 50 percent mortality among gynecological cancers. In early stages, this cancer is usually asymptomatic or symptoms may be equivocal so neither patient nor physician can diagnose it. In this study we report an ovarian lymphoma with acute abdominal presentation in 39 years old woman.Case reportA 39 years woman was admitted to Nekooei hospital in Qom because of abdominal pain and signs of peritonitis. At the time of admission pain pattern was colicky but it became constant in accompany to nausea and mild fever. In the early physical examination she showed generalized tenderness in abdomen without rebound and guarding. After several hours, abdominal signs got worse. Vital signs showed no significant abnormality. Ultrasonography was ordered for further evaluation. Finally patient was made ready for operation with primary diagnosis of ruptured ovarian cyst. Surgery team found out during operation rupture of small intestine due to tumoral growth in the bowel.