Sixty three barley genotypes including 28 cultivated (Hordeum vulgare L.) and 35 wild (H. spontaneum C. Koch) genotypes collected from a wide geographic area of Iran were compared for salt tolerance. Plants were grown in 2-kg pots and subjected to three salinity (NaCI) treatments [0.97 (control), 9.2 and 17.3 dS m^-1] in a greenhouse, in a factorial experiment with complete randomized design and three replications. During vegetative growth, shoot Na^+, K^+, K^+/Na^+ and proline contents were measured for all genotypes. Plant traits including the plant height, length of spikes, number of spikes per plant, fresh weight and dry matter and grain yield per plant were also measured. There was a very wide variation in salt tolerance of the genotypes with regard to Na^+, K^+/Na^ and proline content. In general, tolerant genotypes with better agronomic performance, contained lower Na^+ and a higher amount of proline compared to non-tolerant ones and these two parameters were significantly and negatively correlated (r=-0.62, P<0.01). Salinity tolerance, tolerance index (ratio of grain yield in saline media to grain yield in non-saline media). Was highly negatively correlated with Na^+ (r=-0.9, P<0.01). Therefore, Na^+ and proline content of the genotypes are two criteria which can be used for indirect selection for tolerant genotypes in bleeding programs.