Is spite of wide uses of the Allium leaves, there are paucity of information about the composition of essential oils in the fresh leaves. In this study, composition of essential oil is evaluated in four species of the genus (Subgen. & Sect. Allium) using gas chromatography mass spectrometry (GC-MS). The results showed that the quality and quantity of the essential oil compositions were different in the studied species. Totally, 127 compounds were detected, among those, 68, 21, 29, 31 were found in A. fistulosum, A. iranicum, A. ampeloprasum, A. sativum, respectively. The main compounds in A. iranicum were Trisulfide, dipropyl (34%), 1, 2, 4-Trithiolane, 3, 5-diethyl (16%) while Diallyl disulphide (46%), Trisulfide, di-2-propenyl (11%) in A. sativum, Trisulfide, dipropyl (13. 43%), Diallyl disulphide (10. 38%) and Disulfide, dipropyl (8. 93%) in A. fistulosum and n-Hexadecanoic acid (11. 74%), Disulfide, dipropyl (10. 38%) and Trisulfide, dipropyl (8. 26%) in A. ampeloprasum. Overall, these results could be considered in future breeding programs of Allium species.