Researcher Bulletin of Medical Sciences
Year:2016 | Volume:21 | Issue:1 (109)|Papers Count:9

Background and Aim: Dehydroepiandrostenedione is used for ART methods in women with poor ovarian response. The present study had been performed for assessment therapeutic impacts of DHEA before ART therapeutic procedures on pregnancy rate in infertile women with poor ovarian response.Materials and Methods: In the present double-blinded randomized clinical trial, 120 infertile women with poor ovarian responses in ART procedures were included. Study participants randomly allocated into the trial and control groups. In the trial group, 60 women received DHEA for 1.5 months and in the control group, 60 matched women received placebo during the study. Same ART procedures were performed for both groups after passing study time. Beta-HCG was checked for infertile women two weeks after ART procedure, and two weeks later sonography was performed for assessing number of pregnancy sacs.Results: Finally, six pregnancies had been performed in the study. There was a significant difference between pregnancy rates between two study groups.Conclusion: DHEA usage in the trial group can cause increase in pregnancy rate among women with poor ovarian response.

Background and Aim: Vascular Endothelial Growth Factor (VEGF) and its kinase insert domain receptor (KDR) are crucial for angiogenesis and embryonic development. However, limited information is available regarding the role of the VEGF system, especially its KDR receptor, in recurrent pregnancy loss (RPL). This study aimed to investigate genetic association between VEGF and its receptor gene (KDR) polymorphisms with idiopathic RPL.Materials and Methods: In this case-control study, 50 women who had experienced at least two consecutive spontaneous miscarriages and 50 controls with at least two live births without a miscarriage were included. 2578C/A polymorphism for VEGF gene and Q724H polymorphism for KDR gene, were genotyped using Tetra-Primer ARMS-PCR and PCR–RFLP methods respectively. Achieved results were analyzed statically by the SPSS-16 and chi square methods.Results: The frequency of AC, CC and AA genotypes of VEGF polymorphism in the control and case groups was computed 56, 20, 24% and 26, 46, 28%, respectively (p<0.003). Furthermore, for KDR polymorphism AA, AT and TT genotypes for control 42, 42, 16% and in the case group were detected 30, 68 and 2% (p<0.009).Conclusion: It seems that 2578C/A and Q472H polymorphisms in genes VEGF and its receptor gene (KDR) are associated with idiopathic RPL in Iranian women.

Background and Aim: Recently psychology researchers have focused on the factors that mediate the relationship between attachment and depression. Due to complications associated with ignoring these mediating factors in the etiology of depression diagnosis and treatment of depression. Therefore, in this study through the standard path coefficient, the relationships between attachment styles and basic psychological needs with depression are investigated. The aim of this study is to evaluate the mediating role of the basic psychological needs satisfaction in the relationship between attachment and depression.Materials and Methods: This study was descriptive and 290 students from the Faculty of Psychology and Educational Sciences of Allameh Tabataba'i University were selected. Indicators of basic psychological needs including autonomy, competence and belonging were measured by the Basic Psychological Needs Satisfaction Scale (BPNS-general version), indicators of ambivalent attachment and avoidant attachment were measured by the adult attachment inventory, and indicator of depression was measured by the center for Epidemiological Studies-Depression Scale (CES-D), and the path coefficient were analyzed using path analysis to assess the theoretical model.Results: There was no correlation between the five indicators with depression (r<0.7). The correlation between avoidant attachment with depression was 0.15 but through basic psychological needs satisfaction was 0.35 and the correlation between ambivalent attachment with depression was 0.26 but through basic psychological needs satisfaction was 0.45 (p<0.01).Conclusion: It seems that the basic psychological needs satisfaction moderate the relationship between attachment avoidance and depression. More research in this field is recommended.

Background and Aim: Existing research was conducted in year 1392 regarding the prevalence of ADHD and BPD. Their known complication and some reports based on effectiveness of group emotion regulation training based on process model of gross on use of reappraisal and suppression strategies in externalizing disorders.Materials and Methods: Existing research was conducted in clinical trial and in the context of pre-post-test semi-experimental design with control group. 40 female students with age ranged 15-17 were assigned in 4 groups by multistage random sampling, by the help of standard tools such as SNAP-IV scale and borderline personality inventory (BPI) and then according to screening and diagnostic interviews were conducted by a clinical psychologist and psychiatrist.Results: Data analysis using ANCOVA and follow-up tests show that after emotion regulation group training based on gross model, the mean score of suppression and reappraisal in ADHD experimental group to BPD experimental group increased and decreased significantly (p<0.05).Conclusion: It seems group emotion regulation training based on gross model is effective on use of reappraisal and suppression in ADHD and BPD adolescent girls.

Background and Aim: Anxiety is one of the important disorders of mental health in children and the adolescents’ period, which is influenced by various endogenous and environmental factors. The aim of this study was a comparative survey of anxiety level in primary school children in Gorgan in 2013.Materials and Methods: In this descriptive-analytical and cross-sectional study, 745 male and female primary school students in public and private schools in Gorgan were selected using a combination sampling (stratification and clustering). For data collection Spence Children's Anxiety Scale (SCAS) included 38 questions were used. The collecting data were analyzed using SPSS-21 statistical software and chi-square and t-tests. p-values of less than 0.05 were considered as the significance level.Results: Anxiety disorders in the studied sample were 14.5 percent. The most common disorders were obsessive-compulsive disorder with 18, social phobia 17.6 and separation anxiety 17.6 percent and the fear of open spaces had the lowest prevalence. The results showed that according to Spence Children’s Anxiety Scale total score for the studied sample was 22.74±12.72. The prevalence of anxiety disorders in children with unemployed mothers with lower educational level in female, in families with fewer children and public school students were more.Conclusion: Anxiety disorders are the most prevalence disorder among children and adolescents which have a significant effect on their functioning. This study also underlines the issue that instant identification and treatment of this disorder should be one of the considerations and activities of child mental health specialists. The best place to start screening and assisting these children is primary schools.

Background and Aim: Contamination of soil and water resources with selenium oxyanions is a serious public health and environmental threat. Long-term exposure to high selenite concentrations can result in skin disorders, hair loss, liver and kidney necrosis, breathing problems, deformed nails and cell death. In this regard, isolation and identification of indigenous selenite-resistant yeast strains can be used as safe and natural catalysts in the bio-remediation of the toxic selenite (SeO3-2).Materials and Methods: In this experimental study, collected wastewater effluent and activated sludge samples are cultured into the selective Rose Bengal Chloramphenicol Agar medium containing selenite for selective screening of target strains of yeast. Agar dilution method and disk diffusion testing was performed to determine the intrinsic tolerance isolated yeast strains to selenite. The doubling time and the percentage of growth inhibition of the resistant isolated yeast strains in the presence of selenite were determined from standard growth curves. A colorimetric method using 3, 3-Diaminobenzidine hydrochloride has been developed for the microbial selenite removal. Phenotypic and phylogenetic analyses were used in the characterization of the potent yeast strain.Results: Confirmation of the results from selenite tolerance pattern was performed on turbidity growth curves of the isolated yeast strains. The results showed that the strain se29w, isolated from waste rubber plant, exhibited maximum tolerance of 18 g/l to selenite. The strain se29w was classified as Candida tropicalis (GenBank accession number KR150680). Reduction of selenite to elemental red selenium was also observed in the strain se29w. Based on the results of selenite removal experiments, the resting cells of C. tropicalis se29w is capable remove 92.5% of selenite with initial concentration of 1 g/l at 30 C, pH 7.2, agitation 180 rpm, 20 g/l of wet biomass and after 60h incubation.Conclusion: The present research is the first report on the removal and reduction of selenite with C. tropicalis. The current project have proposed a variety of screening methods for identifying targeted microbial catalysts for bio-remediation of selenite and to improve environmental and human health.

Background and Aim: Q-fever is a zoonotic disease caused by Coxiella burnetii, a small obligate intracellular gram-negative bacterium that is prevalent throughout the world. Cattle, sheep and goats are the main sources of human infection and shed organisms in their milk. Therefore, consumption of raw milk may be source of infection. This study was conducted to determine the prevalence rate of Coxiella burentii in bulk tank milk (BTM) samples, which collected from rural dairy cattles in Shahrekord.Materials and Methods: This cross-sectional study was conducted from January to May 2014. Totally, 50 bulk tank milk samples were obtained from rural dairy cattles and were tested for C. burnetii by Nested-PCR assay.Results: In this survey, 16 out of 50 (32%) cow bulk tank milk samples were positive for presence of Coxiella burnetii. The prevalence rate of C.burnetii in winter and spring seasons was 36% and 28%, respectively.Conclusion: These results indicated that cow milk can be one of the potential sources of C. burnetii in this area of Iran.