This research was conducted in order to study of different levels of zinc sulfate and manganese sulfate micronutrient fertilizers, as a combined form, on three wheat cultivars in irrigated conditions in Abestan, (a part of Zagheh region located in Khoramabad city) during 2008-2009. The experiment was accomplished in a factorial form in a randomized completely block design (RCBD) with three replications. Treatments with three fertilizer levels included control treatment of no fertilizer (F1), zinc sulfate with 25 kg /ha plus manganese sulfate with 45 kg/ha (F2), and zinc sulfate with 50 kg/ha, plus manganese sulfate with 90 kg/ha (F3) in the soil- applied form on three wheat cultivars in irrigated conditions from cold regions namely Bahar (V1), Shiraz (V2) and Pishtaz (V3). The treatments were applied at the time of planting.Measured traits, included zinc and manganese contents of grain, protein percentage, protein yield and grain yield.Results indicated that maximum and minimum contents belonged to treatment F2 in Shiraz cultivar and control treatment in Bahar cultivar with 29.32 and 23.67 mg/kg respectively. On the other side, manganese contents in seeds indicated its most effect on treatment F3 in Bahar cultivar, with 67. 77 mg/ kg. The lowest amount was in control treatment in Bahar cultivar with 52. 33 mg/ kg. Besides, the most protein percentage of grain was attributed to treatment F3 in Pishtaz cultivar with 18.66 percent and the lowest, belonged to control treatment in Bahar cultivar with 12.41 percent. The highest protein yield in grain was attributed to the fertilizer treatment F2 in Shiraz cultivar with 1247 kg/h, and the lowest was attributed to fertilizer treatment (control) in Bahar cultivar with 840 Kg/h. It should be cited, that the highest grain yield belonged to fertilizer treatment F2 in Shiraz cultivar, with 7.383 ton/h, and the lowest belonged to control fertilizer treatment in Bahar cultivar with 5.794 ton/h.Therefore, the results indicated that with applying fertilizer treatments in a combined form in different amounts, an increasing trend could be observed in zinc and manganese contents in seeds of all cultivars used in the experiment, and in the case of protein yield and grain yield, they had direct relation, because the decrease or increase of each one will have a positive or negative effect on another.