This experiment was conducted during 2005 – 2006 in Agriculture Research centre of Jiroft and Kahnouj to determine the effect of water stress on morphological characters of alfalfa cultivars. Using a split plot design based on Randomized Complete Block Design (RCBD) with three replications. The three levels of water stress (A1=severely stressed, A2=moderately stressed and A3=least stressed) was main plot factor and five cultivars of alfalfa (B1=Yazdi, B2=Nikshahri, B3=Rangar, B4=Bami and B5=Baghdadi) were arranged in sub plots. In each cut, total fresh and dry forage weight, number of stem, LAI, number of internode, plant height, leaf stem ratio, leaf number, internode length and protein were measured. The results showed that the effect of water deficit stress for morphological characters especially internode length were significant. By inreasing water stress the fresh and hay forage, plant height, numbers of stem, LAI, internode length and number of node by increasing water stress were decreased.