In order to evaluate the effect of different nitrogen amounts and rows spacing on morphological and medicinal attributes of purple coneflower (Echinacea purpurea L.), an experiment was carried out in Isfahan Agriculture Research Center. The experiment was as split plot based on completely randomized block design with three replications. N fertilizer levels including 0, 75, 150 and 225 kg/ha considered as main factor and different rows spacing 15, 30 and 45 cm lacated in sub factors. Analysis of variance showed that the effect of different nitrogen levels on seed yield per plant, root dry yield, root fresh weight, shoot fresh weight, number of leaves, number of flowering branches, flowering branch length, plant height and flower height was significant. Number of flowers per plant, plant height, leaf number, shoot dry and fresh weight, root dry and fresh weight, flower height, leaf area, seed weight and seed yield per plant were raised with increasing nitrogen fertilizer up to 225 kg/ha. The experimental results showed that also the number of flowers per plant, number of branches, plant height, flower height, number of leaf, shoot fresh and dry weight, root fresh and dry weight, leaf length, leaf area and seed weight were maximum in 45 cm row spacing. Clorogenic acid amounts in shoot were variable between 3.56% (Control+15 cm rows spacing) and 4.84% (150 kg/ha nitrogen+30 cm rows spacing).