This study was carried out during June to October 2012 in the laboratory of the department of the horticultural science of islamic azad university, branch Garmsar to find a proper treatment to increase the shelf-life of strawberry fruits (Fragaria annanasa L. cv Camarosa). The fruits were collected from a greenhouse at Hashtegerd and were brought to the lab immediately. In this study the effect of different concentrations of spermine include 0, 0.5, 1, 2, and 4mM on postharvest strawberry fruit plants were compared. Fruits were placed in the solution for 5 min and then were dried and kept in refrigerator with a temperature of 5o. Firmness, soluble solids, titratable acidity, pH, fruit juices, fruit quality and storage life were measured in the early days, fourth, eighth and twelve after storage. Significant increases of spermine storage life and fruit characteristics of strawberry was so in 7 days storage life of fruits, while immersed in a solution of 2 mM spermidine, the fourteenth day were still marketable. Use of spermidine was significantly protecting other fruit quality traits. The use of 2 mM spermine significantly prevented loss of fruit firmness during storage and keep the fruit firmness. Fruit quality was lowest in the control group gradually increased polyamine concentration increased to 2 mM and higher quality of life was reduced to 4 mM. In comparison polyamins, spermine test results showed the weak.