Many studies have been undertaken to evaluate the antioxidant activity of different essential oil and plants extract recently, which emphasize the ability of these compounds to inhibit growth of wide range of microorganisms and spoilage agents. Therefore, in order to evaluat the antioxidant activity of seed extract of 15 grape cultivars (Fakhri-E-Kashmar, Shoghali-E-Neishaboor, Sahebi-E-Neishboor, Mayemish-E-Birjand, Sefidangoor-E-Ghoochan, Rokhsiah-E-Mahvelat, Lalghermez-E-Mahvelat, Amirozom-E-Rezaie, Moghanai-E-Mashhad, Amirgile-E-Rezaie, Sabzeh-E-Mashhad, Tabarzeh-E-Rezaie, Shahani-E-Rezaie, Loflofi-E-Lotfabad and Samarghandi-E-Lotfabad), an experiment was conducted based on completely randomized design with 4 replicates during 2009-2011. The antioxidant capacity of seed extract was assessed by DPPH method. Results showed a considerably high antioxidant capacity in all tested cultivars. The minimum and maximum values of 12.53 and 90.2 were observed in Sefidangoor-E-Ghoochan and Shoghali-E-Neishaboor, respectively. The cultivars Amirozom-E-Rezaie, Amirgile-E-Rezaie, Moghanai-E-Mashhad, Samarghandi-E-Lotfabad and Shahani-E-Rezaie, at the next ranks, had the maximum antioxidant capacity. So that, grape seed extract, which is a good natural and beneficial material for human health, can be recommended as a suitable source of antioxidants.