Today, the emphasis is on stable agricultural, the long-term stability of crop production with minimal impact on the environment. The use of biological fertilizers has been considered in recent years, with the purpose of eliminating or reducing many environmental impacts irreparable, caused by indiscriminate use of chemical-based agricultural ecosystems. To study the effect of Mycorrhizal and Azotobacter biological fertilizers on growth and yield of red bean, an experiment in crop year 2016 in Lorestan Beiranshahr, a factorial experiment was arranged in randomized complete block design with four replications. Parameters studied: mycorrhizal fungi at two levels (inoculated and no inoculated), inoculated with Azotobacter bacteria at two levels: (inoculated and non-inoculated) and red bean (Beiranshahr landrace, Akhtar and Goli Cultivars). The results showed the effect of three factors were significant at probability 1 percent level, plant height, number of pods per plant, pod length, number of seeds per pod, number of seeds per plant, seed yield and seed protein yield; And the interaction of three factors (Azetobacter×Mycorrhiza×varieties) on plant height, seed yield and seed protein yield at 5 percent probability level. seed yield in Beiranshahr landrace, Akhtar and Goli Cultivars, increased 56. 5, 19. 1 and 43. 2 Percent Respectively. The highest seed yield was obtained from the combined effect of Inoculation Azotobacter×Mycorrhizal inoculation×Goli Cultivar (298. 90 kg. ha-1) plants with form the progressive and unlimited growth. According to the findings of this study, the use of biological fertilizers containing arbuscular mycorrhizal fungi alone or along with Azotobacter crocococcus can be recommended in red bean cultivation.