JOURNAL OF DEVELOPMENTAL BIOLOGY
Year:2019 | Volume:11 | Issue:1 |Papers Count:8

Introduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyapatite. The aim of this study was to evaluate the biocompatibility effect of PLGA/HA scafffold on osteoblast differentiated from hEnSCs. We aimed to evallute the adhesin of cells on scaffolds. hEnSCs were isolated from human endometrium tissue and induced the osteogenic differentiation, then the differentiated cells were cultured on PLGA/HA synthetic scaffolds. A nanocomposite scaffolds based on PLGA/HA were fabricated by electrospinning methode and behavior of differentiated osteoblast cells was evaluated after seeding cells on this scaffold. Osteogenesis was investigated in terms of attachment, alkaline phosphatase activity and gene expression. Morphogenic of cells in scaffold was evaluated by SEM and biocampatibility of this scaffold was confirmed by MTT assay. The alkaline phosphatase activity confirmed osteoblast differentiation. SEM studies showed that the surface properties of scaffold were desirable and the cells had the ability to attach and proliferate better on the nanocomposite scaffolds. It has been shown that the nanocomposite scaffolds have appropriate properties to support the attachment of differentiated osteoblast cells.

Ocimum basilicum L. from Lamiaceae is used as a herb, spice and fresh vegetable. Since medicinal plants have different reactions to drought stress in the production of effective compounds, Diagnosis of medicinal plants growth under different irrigation conditions and drought stress can be a guide for growing plants in dry and low water conditions. Therefore, in order to evaluate the effect of drought stress on morphological characteristics and essential oil content, especially linalool, in different stages of plant growth of Basil plant, a completely randomized factorial design was carried out in 3 replications. The experimental treatments included three levels of dryness (no stress, mild stress and severe stress) in potted conditions, in vegetative, pre-flowering, flowering and fruit-growing stages. The results showed that drought stress had a significant effect on evaluated characteristics. So, with decreasing of soil moisture content, plant height, number and leaf area, decreased in all stages of growth and Stem diameter increased at all stages, especially in severe stress and root length, increased during flowering stage. In reproductive stages, drought stress caused a significant reduction in the number of flowers than the control. Extreme stress in flowering and fruiting stages has had decrease in the amount of essential oil production, especially linalool. However, mild stress showed increase in the production of these compounds in both steps. The highest percentage of essential oil was related to mild drought stress at flowering stage and the highest amount of linalool was due to mild drought stress in the fruiting stage.

Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal side effects. This study was evaluated effects of Hydroalcoholic extracts Ruta graveolens L. against of HeLa cell. Material and Methods HeLa cells were cultivated in Dulbecco’ s Modified Eagle’ s Medium (DMEM) supplemented with 10% fetal bovine serum. Preparation of extract were done by maceration technique. This study were assessed the effects of different concentrations (250, 500, 750, 1000 and 1500 μ g/ml) of Ruta graveolens L. on the proliferation of HeLa cells at 24, 48 and 72 hours after treatment of cells with extract and medium containing 1% serum by Trypan blue and MTT methods. Finding The cytotoxic concentration 50% (CC50) of the extract was determined on HeLa cell 1500 ug/ml. The results showed that concentrations of 1000 ug/ml of Ruta graveolens L. extract, after 72 hours showed a significant decrease in the proliferation of HeLa cells compared with control. Conclusion: The results of this study showed Ruta graveolens L. extract can inhibit the proliferation of HeLa cells. Keyword: Cytotoxic effect, Ruta graveolens L. extract, HeLa cells.

To replace fertilizers from nanotechnology with chemical fertilizers much effort is being made. Fennel is a medicinal plant which has a special place in the pharmacy and food industry. In this study, the effect of magnetized water, nitrogen and nano-nitrogen fertilizers on germination of seeds and growth parameters of fennel was studied. Determination of germination percentage showed in both types of irrigation, using nitrogen and nano-nitrogen fertilizers significantly reduce the percentage of germination. Maximum germination percentage was obtained in irrigation with plumbing water or magnetized water without using nitrogen and nano-nitrogen fertilizers. The highest average number of shoots per plant was obtained in the treatment of magnetized water alone or treatment with plumbing water together with nitrogen fertilizer. The results showed that in both types of irrigation, nano-nitrogen significantly reduces the number of shoots. But it increases the stem's length. Irrigation with magnetized water along with nitrogen and nano-nitrogen fertilizers resulted in a significant increase in the average number of leaves. The results showed although the treatment of plants with magnetized water and nano-nitrogen significantly decreases the shoots numbers but it has a significant effect on stem growth and leaves number. GC-MS analysis of essential oils of petals indicated that the four main compositions in all treatments were limonen, fenchone estragole, and anethol in all treated plants.

Garlic has been known worldwide as a dietary constituent with many pharmacological effects in humans for centuries. The aim of this study was to compare the cytotoxicity effect of the aqueous extract of garlic on breast cancer cells (MCF-7) and non-cancerous normal cells (L929). After culture and treatment of cells with different concentrations of extract for 24, 48 and 72 hours, the cell morphology was investigated by invert microscopy and the toxic effect of the extract was determined by MTT (3-4, 5 dimethylthiazole 2-yl 5, 2-diphenyl tetrazolium). Microscopic observations showed no change in both cell lines within 24 hours. These changes appeared after 48 and 72 hours in cancerous cells and increased after 72 hours, but no significant changes were observed in healthy cells. At the same time, the results of MTT showed that the mortality rate was significant at high doses at 24 and 48 hours (P <0. 001), and in 72 hours it was very intense and survival rate reached 48% at the highest dose. However, at 24 and 48 hours, we did not see significant death in non-cancerous cells, but at higher doses of 4000 μ g/ml in 72 hours, death increased significantly (P <0. 001) but did not exceed 19%. Therefore, aqueous extract of garlic has a greater effect on breast cancer cells than normal cells, and can be used to prevent cancer or as a supplement to chemotherapy in the future.

For this purpose, this study was carried out with the aim of the best treatment for breaking the dormancy and improving the germination characteristics of white currant seed under the influence of various chemical and physical treatments. (Astraglus gossypinus) Fisher. Is one of the most valuable and productive plants of the highest quality gum, which is very important in protecting the soil and economy of the country. In order to investigate the effect of silver nanoparticle (AgNPs) and Gibberellic acid (GA) on (Astraglus gossypinus) germination, a factorial experiment was conducted in a completely randomized design (CRD) with 4 replications in 1397. Experimental treatments consisted of priming with gibberellic acid in 4 levels (0 as control, 100, 150 and 300 ppm) and silver nanoparticles in 4 levels (0 as control, 0. 05, 0. 1 and 0. 2 W/V) for 4 hours at 25 ° C. The results of the experiments showed that gibberellic acid, silver nanoparticles, and interactions of treatments at 1% probability level on all studied traits including germination percentage, root length, stem length, germination coefficient, and relative water content, chlorophyll a, b and total chlorophyll were significant. Also, the use of 100 ppm gibberellic acid increased the root length by 25%, but with increasing gibberellic acid concentration, root and shoot length decreased compared to control.

Breast cancer is a multifactorial disease in which genetics, hormonal factors and the interaction between individuals and the environment contribute to it. In the present study, we try to compare the effect of lime leaves extract and S14161 small molecule on apoptosis of MCF-7 breast cancer cells. Apoptotic effects of S14161 and hydroalcoholic extract of Lime leaves on MCF-7 cells were investigated using MTT method, the morphology of cells with acridine orange staining using the fluorescent microscope. The results showed that the S14161 and Lime leave extract decreases the survival rate and proliferation of MCF-7 cells in a time and dose-dependent manner. Based on the results of the MTT test, 10 μ M concentration of S14161 and 6 mM concentration of Lime extract was determined within 24 hours as IC50 concentration of MCF-7 cells. Comparison of Lime extract and S14161 showed that the amount of small molecule cytotoxicity was higher than that of Lime leaves extract and there was a significant difference between survival percentage of cancer cells treated with S14161 and cells treated with lime leaves extracts on day 1, 3 and 5 (P <0. 05). Also, there were significant morphological changes such as shrinkage and increasing size of vacuoles, cytoplasmic loss, nucleus pigmentation and fragmentation of chromatin in treated cancer cells. The specific effect of this inhibitor on cell growth, as well as the lack of observation of toxic effects to high concentrations, has been described as a major strategy for treating breast cancers.