Introduction: Coronary artery disease (CAD) is a leading cause of death in the developed and undeveloped world today. It is well established that increased total, LDL-cholesterol, and triacylglycerol concentrations, as well as decreased HDL-cholesterol concentrations, are strong independent predictors of CAD (1). Aerobic exercise is believed to reduce the risk of cardiovascular disease partially through increasing serum levels of high-density lipoprotein cholesterol (HDL-C). However, this effect varies considerably among exercise intervention studies. therefore, we considered the effect of 8 week endurance training of different duration son resting levels of HDL, HDL2, HDL3, TG, TC in plasma.Materials and methods: 50 Wistar male rats (6-8 week old) with mean weight of (189.98±6.589) grams were used. The rats were randomly divided into 5 groups (each group of 10 rats) including 3 experimental groups with different durations of 30, 60 and 90 minutes, abasic control group (in the cage) and basic group 2 (sham). The experimental groups were trained for 8 weeks, 5 days a week, each day with the speed of 20 m/min with different durations of 30, 60 and 90 minutes. 72 hours after the last session of training the rats were fed and killed (4 hours ago the food was taken from the rats' cage). Then, plasma was collected.The levels of HDL, HDL2, HDL3, LDL-C, TG and TC were measured by sedimentary and enzymatic methods. The data analysis was performed through statistical measures of ANOVA and LSD by using SPSS (version 16) software at significant levels of 5%.Results: No significant effect was found in levels of HDL, HDL2, HDL3-C, TG, LDL-C, VLDL-C, TC/HDL-C, LDL/HDL-c and HDL2-C/HDL3 between any of these groups after 8weeks endurance training (P=0.05). However, the results illustrated TC levels decreased significantly after 8 weeks training in 30 min and 60 min groups in contrast with control and sham groups (F=6/566, P<0.000).Conclusion: The present study showed that 8 week Endurance training with different durations has no significant effects on resting levels of HDL, HDL2, HDL3, TG, LDL-C, VLDL-C, TC/HDL-C, LDL/HDL-C and HDL2-C/HDL3. But TC levels decrease significantly after 8 weeks of endurance training.