Background and Aim: Breast cancer is the most common malignancy of women. Given the importance of breast cancer and the necessity of screening studies in the community, as well as the important role of female teachers, the present study aimed at determining Knowledge, attitude, and practice of female teachers regarding breast cancer screening in Birjand.Methods and Materials: In this descriptiveanalytical study, 400 female teachers from schools in Birjand were selected using multistage sampling method. After explaining the study objectives and encouraging them to collaborate, questionnaires were completed through interviews. The obtained data was analyzed by means of SPSS software using chi-square, Kruskal-Wallis, and Mann-Whitney tests atr the significant level P=0.05.Results: Out of 400 participants, 307 (76.8%) had Bachelor degrees and 386 (96.5%) were married. Mean age of the teachers was 38.22±6.48 years. Only 127 (31.8%) used to do regular breast self-examinations (BSE). Mean score of knowledge about breast cancer, BSE, risk factors, and symptoms was 2.10±0.67, 1.10±0.77, 8.05±3.34, and 6.80±2.73, respectively. There was no significant difference with respect to age, marital status, and history of breast cancer in first-degree relatives and acquaintances. Mean knowledge score of cancer and its risk factors was higher in women with higher education (P=0.01, P=0.007, respectively). Mean score of perceived susceptibility, severity, perceived benefits and barriers was 12.74, 6.31, 39.68, and 20.78, respectively. There was no significant difference in terms of education and history of breast cancer in relatives. Mean score of perceived susceptibility was significantly different in different age groups (P=0.009).Conclusion: According to the results of the present study, education about breast cancer screening, particularly training to improve women’s practice is recommended.