The present research has been carried out to investigate the effect of spraying of Origanum vulgare and Thymus vulgaris L. extract on quantitative and qualitative characteristics of sesame (Naz cultivar) under drought stress conditions. Performed as a split-plot factorial in two cultivation seasons of 2016-2017 and 2017-2018, it is as a randomized complete block design with three replications. Its treatments include two different irrigation regimes (every 15 and 25 days), three levels of Origanum vulgare (0, 40%, and 60%), and 3 levels of Thymus vulgaris L. (0, 10%, and 20%). Results indicate that the maximum leaf area index (3. 76 and 2. 93), number of capsules per plant (38 and 22), number of seeds per capsule (54 and 47. 75), and grain yield (197. 92 and 141. 28 gr m2) in both control and stress conditions have been associated with 60% Origanum vulgare and 20% Thymus vulgaris L. extract. Moreover, it has been revealed that water deficit reduces oil content, increasing protein content and activity of catalase, peroxidase, and superoxide dismutase enzymes. The use of 60% Origanum vulgare and 20% Thymus vulgaris L. extract, under suitable irrigation conditions increase the activity of catalase, peroxidase, and superoxide dismutase enzymes by 11. 24%, 12. 65%, and 11. 24%, respectively, compared to the control. In contrast, these indices are increased by 36. 36%, 36. 35%, and 32% under stress conditions. Therefore, it could be stated that under dry conditions, the use of Origanum vulgare and Thymus vulgaris L. extract can induce tolerance in the plant in response to drought conditions.