JOURNAL OF VETERINARY CLINICAL RESEARCH
Year:2013 | Volume:4 | Issue:1 (13)|Papers Count:8

Peptic ulcer is a common disease in veterinary and human medicine. Protective factors in gastrointestinal tract contain: mucosal defense, prostaglandin G, local circulation, mucous, Antioxidants (vitamin E, vitamin C, melatonin, Superoxide dismutase). Ulcerogenic factors in gastrointestinal tract contain: HP, acid, pepcin, stress, Genetic, free radicals, NSAIDs, duodenogastric reflux, virus, zollinger- ellison syndrom. Indomethacin is a NSAID drug. Omeprazole, rabeprazole and pantoprazole are Proton Pump Inhibitor (PIP) drugs.This study have performed on 6 groups of rats: saline, indomethacin, omeprazole (10mg/kg)+indomethacin, omprazole (1mg/kg)+indomethacin, rabeprazole (10mg/kg) + indomethacin, rabeprazole (1mg/kg)+indomethacin. All rats were killed 4 hours after the last injection of indomethacin and stomachs were prepared for calculation of j.score and histopathological study.In indomethacin group, injection of indomethacin was associated with significant increased in j.score compared with saline group. Injection of omeprazole and rabeprazole, was associated with significant decrease in j.score compared with indomethacin group.Injection of rabeprazole, was associated with significant decrease in j.score compared with omeprazole group. Histopathological results confirmed j.score results.

Deposition of iron particles and pigments may have different effects on affected tissues. For differential diagnosis of each substances deposited in cells and tissues, examiners have to use specific methods for each material. This diagnosis usually based on the specific staining methods of affected tissue. Two different specific Iron staining methods Gomori and Perl’s Prussian blue were compared in this study. Among this survey 860 slaughtered Caprine were investigated histopathlogically and macroscopic Iron pigments deposition grossly found in 8 of these animals. This accumulation was in renal cortical area pertaining to cloisonné kidney disorder. After fixation in formalin buffer 10% solution, samples dyed with two specific Iron staining methods: Perl’s Prussian blue andgomori. Comparison results showed significant differences between quality and quantity of prepared histopathologic samples. For more scientific investigation, computerized analytic software Scion Image used for comparing prepared images of two different staining methods. The received data used in analytic software SPSS for statistical analysis. The results confirmed exactly more efficiency of Perl’s staining method compared to Gomori iron staining method. These superiorities were in more effective coloration of Iron pigments, peripheral cells and more contrast and resolution of stained tissues.

Liver is the main organ in fat metabolism and its ability in storing and metabolism of extra fat available in feedstuff is, in rainbow trout this ability is limited so the extra fat of feedstuff can be piled up in liver and lead to Lipoidosis. In this study 120 rainbow trout from western Azerbaijan’s rainbow trout farms during fall and winter were randomly selected and put into four different weight groups (below 50-50to150-150to250and above 250gr) for the analysis of Lipoidosis disease. after biometric experiments the fishes were autopsied and after sampling and tissue stabilizing in formaldehyde the pathological sections of 135 samples of suspicious liver were prepared in 5micrometer diameter and colored by two methods of PAS and H&E (for determining fat). In livers affected by Lipoidosis the penetration of fat into hepatocyte was seen as clear vacuoles so that after PAS coloring their fatty nature was confirmed because they were not colored. The results show that by an increase in weight. (weight group above250gr) the rate of Lipoidosis increase too, the study histopathological sections in affected livers other damages like focal Lymphocytosis low to acute bleeding, dilation of central veins and increase of melanomacrophage focuses as well as Lipoidosis were seen. By noticing the high outbreak of this disease in weight group above 250gr whish has also the probability of economic losses.

Microencapsulation as one of the most modern methods has remarkable effects on probiotic survival. The objective of this study is to evaluate the survival of free and microencapsulated probiotic bacteria in simulated human gastrointestinal condition.Lactobacillus casei (PTCC 1608) and Bifidobacterium bifidum (PTCC1644) were encapsulated with calcium alginate, resistant maize starch and inulin via emulsion technique and incubated in simulated gastric juice (pepsin included, pH=1.5) and simulated intestinal juice (pancreatin and bile salts included, pH= 8) for 2 hours at 37 oC. The morphology and size of microcapsules were measured by SEM technique and optical microscopy. The results showed that the survival of microencapsulated probiotic was increased significantly in simulated gastro-intestinal condition (P<0.05). Resistant maize starch was played a significant role in the protection of probiotic bacteria in simulated gastro-intestinal condition and did not affect the size of microcapsules. In the emulsion technique capsules are formed in micron range size and the shape of microcapsules was generally spherical.In general, this study indicated that microencapsulation enhanced the survival rate of probiotic bacteria significantly in the simulated human gastro-intestinal condition.

Maintenance conditions of domestic and laboratory animals can lead to wide variations in their metabolism. Individual housing is one of the most effective factor which can affect their metabolism. In this study, the effect of individual maintenance on body weight of 28 mice in both solo and group maintenance group for 4 weeks were studied.The results of this investigation showed, the body weight in individual male & female mice was more than group mice as meaningful. Body weight of individual male mice group, was more than individual female mice group as meaningful that shows more effect of individual life on male mice.Based on these results, it is recommended to have this issue addressed in all the processes of maintenance and breeding of animals that require individual housing.

Gold fish or Pondfish (Carassius auratus), is among the valuable ornamental fish from Cyprinidae family, which compared to other ornamental fish in the world, is sold extensively for low prices, especially in Nowrouz, in Iran. In this study, through colleting fish from Breeding and Pisciculture centers in Urmia city and transporting them to the laboratory of parasitology of Urmia Islamic Azad University in special fish transportation sacks, separation of external parasites from skin, fins, eyes, and gills was done. Among 92 investigated goldfish, 56.52% were contaminated with parasites. In this study 7 different genus were separated. The highest contamination percentage belonged to species Ichthyophthirius multifiliis and followed by Dactylogyrus spp. and protozoa Trichodina spp. Among single hosted trimatodes, Gyrodactylus spp. was also separate. Among multi hosted trimatodes, parasite Diplostomum spathaceum was separated from the eye lens and parasite Diplozoon Nordman was seen in this study too which is the first time to be reported in goldfish in Iran. Among Fish lice parasites, genus Argulus foliaseus was separated.

A 6 Months old holstein female dairy calf was referred to the clinic of veterinary medicine, Ferdowsi university of Mashhad in the spring of 2011. The owner’s chief complaint was anorexia and growth retardation. Clinical examination revealed depression, tachycardia, tachypenia, atonic rumen, weakness, recumbency, increase breath sounds with harshness, cardiac murmur (Left side of the chest, base of heart with mild to moderate intensity), jugular vein distention, submandibular and brisket edema. Haemogram profiles showed an acute infection with degenerative immune system response. Radiological findings were consisted of increased radiopacity and alveolar pattern (air bronchogram) in cranioventral lung lobes. Thoracic and abdominal ultrasonography was performed. Anechoic free fluids in pleural and abdominal cavity were observed which indicated pleural and peritoneal effusion, respectively. According to these findings and a survey on serum biochemical profile, with measuring the concentration of serum troponin I and rule out other causes of edema and ascites, the cor Pulmonale associated with bronchopneumonia was diagnosed. The sick animal was treated by parenteral antibiotic, diuretic and supportive drugs. About 2 weeks after the treatment, clinical examination, radiological and ultrasonographical observations and haemogram profiles indicated complete recovery. Three months after the initial presentation, the calf was visited again and the only problem was growth retardation that may be due to bronchopneumonia outcome.

A 6 month German shepherd dog, 10 kg body weight was referred to the private small animal clinic. History taking and physical examination revealed that the patient was suffering from a recurrent rectal prolapse during 14 weeks and despite multiple treatments (medical and surgical), none of them were not effective. Therefore, a surgical treatment to fix the colon to the abdominal wall was performed. Eight months post surgical, no recurrence of the prolapse has been reported. According to the results, we can conclude that fixing the colon (Colopexy) to the abdominal wall is an appropriate and effective treatment Method for the recurrent rectal prolapses.