The main objective of this research was to identify the barriers to promote female athletes in Khuzestan Premier League. Given the lack of proper tools to investigate the barriers, a questionnaire was first designed and its validity was evaluated in three stages.In the first step, after literature review, semi-structured interviews were conducted with female athletes and a primary questionnaire with 62 items was prepared. After review by the research team, the overlapping items were removed and finally 54 items were identified. In the second step, exploratory factor analysis was used to reduce the number of items and to identify more relevant items. Removing 8 items, 46 items remained and classified in 9 main factors. In the third step, the validity of the structure and test was evaluated by partial least squares method. Confirmatory factor analysis was conducted to verify the validity. The results showed that the 8-factor model fits better than the other models. Accordingly, 8 main factors with 43 questions were identified as barriers to the promotion of female athletes in Khuzestan. According to the ranking of obstacles by Friedman test, community impact with the average rating 5/37 is in first place obstacles.management factors 5.25, gender inequality in sports organizations 5.23, technical factors 5, corruption in sports organizations 4.87, costs and career 4.24, gender attitudes 3.25, and gender and culture 2.80 are in second place to eighth.