The goal of this research is to determine the effect of Sodium Bicarbonate usage on some variables and blood gases and performance among runners at university. In this study, there were two conditions the runners experienced: experimental condition (using 300 ml. gr. of Sodium Bicarbonate for each klg. of body weight) and placebo condition (using 300 ml. gr. of Sodium Chloride for each klg. of body weight), the runners, also, ran on the treadmill on maximal performance and interval activity (one minute for six times– the last time, running a distance of 400 m). There were three stages of blood test at each trial session, on which 2 cc of blood sample were taken from the testee's forearm and the samples were tested. These three stages included Rest Stage (R), Post Placebo or Drug Usage Stage (PS) and Post-Activity Stage (PE). The goal of blood sampling was to determine and compare the selected blood variables such as Bicarbonate Ion (HCO3-), Lactate, PH, Partial Pressure of Carbon Dioxide (PCO2) and Partial Pressure of Oxygen (PO2). Then, these variables were compared with other averages at similar stages of measurement (the meaningful level is considered as 0.05). The results of measuring blood selected variables showed that using Sodium Bicarbonate in experimental test caused a meaningful increase at variables of HCO3- and PCO2. It indicated the increase in tympanic capacity of extra cellular environment. It predicted that this factor will cause increase in maximal performance of the athletes. At the stage of post-activity, the HCO3- decreased meaningfully and also, there were a meaningful increase in PCO2, PH decrease became modified, too but it wasn't meaningful. There was a meaningful increase in Lactate at the post-activity stage. It wasn't a meaningful increase comparing with the two groups. PO2 changed meaningfully between post usage stage (PS) and post activity one (PE), it decreased at post-activity stage (PE), that decrease was less at experimental group. In measuring the record of 400m running at Bicarbonate group, it modified meaningfully which showed more activity in experimental group rather than placebo one. It seems that Sodium Bicarbonate can modify the performance and record among the runners, so, it is recommended that Sodium Bicarbonate usage is a supporting, effective, energetic factor especially in studies benefited from maximal performance and interval activity.