Introduction: Fatty liver disease is the most important reason for chronic liver diseases, from simple fatty liver to advanced stages such as liver fibrosis, cirrhosis and liver cancer. In this study, the effect of moderate intensity endurance exercise and probiotic consumption on MMP 9 gene expression in an animal model of steatosis was investigated. Method: This study is an experimental study of laboratory type. 32 male Wistar rats were divided into 4 groups of 8: healthy group, fatty liver group, fatty liver + probiotic group, fatty liver + probiotic group + moderate intensity endurance exercise and were tested for 8 weeks. Endurance training was done for 8 weeks, 5 days a week, at 18-28 meters per minute. The statistical calculation of this study was done using the one-way analysis of variance test and, in case of a significant difference, the Bonferroni post hoc test to determine the location of the difference between groups was done with SPSS 25 software. Results: Endurance training and probiotic consumption have a significant difference on the expression of the MMP-9 gene in the animal model of fatty liver (P=0. 000) and decrease the expression of MMP-9. Conclusion: According to the results of the study, it seems that endurance training along with probiotic consumption can make patients with fatty liver resistant to the damage caused by this disease.