The purpose of this research, Determination of hematological and biochemical parameters in two species of Acipenseridae, Acipenser stellatus and Acipenser nudiventris In three Age of groups, Fry’ s (2-7 months), Juveniles (1-2 years) and Spanners (7 years). The research at the Institute for International Research sturgeon of the Caspian Sea, in a period of six months (spring and summer) was conducted in 2013. For this reason, 30 numbers of Acipenser Stellatus and 30 numbers of Acipenser nudeventris were used (in each group there were 10 fry’ s, 10 Juveniles, 5 females and 5 males fish). After caudal vein blood vessels, hematological parameters (RBC, WBC, MCV, MCH, MCHC, Hb, PCV, Lym, Mono, Neut, Eos) and biochemical (Serum osmolality, albumin, total protein, glucose, cholesterol, Triglyceride, calcium and magnesium) Determined and studied. The results showed that, RBC, WBC, MCH, MCHC, Hb, Mono, Neut, Eos and total protein, glucose, cholesterol, Triglyceride, calcium and magnesium ions in both sturgeon species (A. nudiventris and A. stellatus) during the 3 groups age difference was statistically significant (p<0/05). But, PCV, Lym, MCV and osmolarity serum, Albumin were only statistically significant difference in the species A. stellatus (p<0/05). Seem to be gender parameters of RBC Mono, and Albumin only in the species A. stellatus and WBC only in the species A. nudiventris has been significant (p<0. 05). A significant statistical difference was estimated in 95% of all data. By investigating the present results, it can be stated that age and species factors in the change of blood parameters are so effective (p<0/05), but gender didn’ t show any effect on the biochemical and hematological parameters. (p>0/05).