The aim of the present study was to investigate the effect of whole body vibration (WBV) on anaerobic performance indexes and flexibility on active men. The participants were 12 trained healthy male with the average age of 23.41±2.1 years old, body mass of 71.16±5.06 Kg, height 178.16±2.94 cm, and body fat percentage 15.55±5.15. The study was conducted in two days with time interval of one week In the first day, 10 min active warm up was carried out including jogging, stretching movements, and cycling on ergometer, Then, several tests was carried out; sit and reach, vertical jump and Wingate Test of 30s, for assessing anaerobic peak power, average power, minimum power and fatigue index percentage. In the second day, participants use the vibration with frequency 30 HZ and amplitude 10 mm was conducted 4 times including 3 min vibration along with 1 min rest lasting for 15 min, then the tests of sit and reach, vertical jump and Wingate Test of 30s was implemented. The results were analyzed by T-test. The results indicated that WBV leads to a significant increase in the amount of vertical jump (P=0.044), anaerobic peak power (P=0.045) and anaerobic average power (P=0.021) in compared with active warm up. In other indexes no significant change was found.