Twenty different cultivars of potato were evaluated for their relative resistance to the Colorado Potato Beetle, Leptinoiarsa decemlineata (Say), (CoL Chrysomelidae) and the mechanism involved at the agriculture research stations of Allarough and Hassan barough in Ardabil in the field during 1997 and 1998. The cultivars were: Fresco, Premiere, Caesar, Idole, Casmose, Desiree, Ajax, Cardinal, Mondial, Remarka, Oblixe, Picaso, Vital, Apollonia, Marfona, Concord, Qaster, Carlita, Draga and Aula. In a laboratory experiment second instar larvae of the beetle were confined on the fresh leaves of different cultivars in a repeated experiment and the mean amount of leaf eaten and the weight gain of the larvae were calculated and used as indices of antibiosis. In a Choice Test experiment that was conducted in the field, the number of adult beetls observed on each of the cultivars was determined 24 hours after their release and used as an index of antixenosis. To delineate tolerance, cultivars were planted in pots in a Randomised Completely Block Design and were infested with an equal number of first instar larvea. screen cage of suitable size was placed over each pot. At the end of the season, the tuber yields was harvested and compared. There was no indication of antixenotic and antibiotic resistance among the cultivars. However a significant difference in percent of reduced yield and damage at infested and noninfested pots was found that indicates the existence of difference in the tolerance of cultivars against the pest. Therefore, it was suggested to further study the genetic basis of the tolerance in order to use in an IPM program of the Colorado patato beetle.