Purpose: The objective of this study was to investigate the alterations of Serum levels of brain derived neurotrophic factor, and oxidative stress following a period of plyometric training in active men.Methods: Fourteen healthy active male students randomly divided into two groups: training (mean age 22.14±1.34 years, height 172.14±3.89 cm, weight 63.42±8.77 kg, BMI 21.42±2.77 kg/m2) and control (mean age 23.85±2.54 years, height 178.57±7.11 cm, weight 71.71±4.53 kg, BMI 22.60±1.90 kg/m2). Training group performed 4 weeks of plyometric training. BDNF, malondialdehyde and superoxide dismutase activity was measured. Paired sample t-test was used to examine differences within groups, and independent t-test was utilized to examine differences between groups. Differences were considered to be significant when p<0.05.Results: In compared within group, BDNF levels of training group did not change significantly (P>0.05) compared with before training, whereas serum levels of SOD and MDA increased significantly (P=0.002, P=0.036, respectively). BDNF and MDA did not change significantly between control and training groups following 4 weeks of plyometric training (P>0.05) but caused a significant increase in serum levels of SOD (P=0.049).Conclusions: The findings of the present study indicate no significant changes in BDNF levels following regular plyometric training. Regarding increased lipid peroxidation index values in within group and lack of any changes in between group, can not accurate conclusion about the possible antioxidant role of BDNF against stress induced by physical activity, so needs to more investigations.