This research tried to investigate and compare the effect of electronic music on speed running of runners and non - athletes of Tehran in moderate level. For this reason, among male runners of Tehran who worked in moderate level and exercised in running field under the supervision of teachers for minimum three months, only 41 (X±SE age 16.5±1.31Y) who exercised in Shahid Shiroodi stadium were selected as sample and as subjects of running test. They were classified in three experimental groups and one controlling group. The groups were harmonized according to the records of pretest. Among the male non -athletes of moderate level in Tehran, 54 male students of technical school in district of education (X±SE age17.39±1.01Y) were selected as subjects. In this plan, after 20 minutes of exercise (1 minute walking quickly, 8 minutes running smoothly, 8 minutes performing active and static tension exercise and 3 minutes interval exercise), speed running test of 100 meters was performed as the pre -test for all non-athletes according to the records of pre - test. Subjects were classified in 3 experimental groups and one controlling group. Each independent group (electronic music with fast and with slow rhythm and with desirable music) accidentally was chosen for each experimental group. After 48 hours, each group listened to the mentioned music through walkman and headphones along with performing 20 minutes of exercise. After this period, a post - test (running 100 meters) was performed. But for controlling group after 20 minutes of exercise without music, post-test was performed. This method was performed over runners. Then, T test was performed to analyze statistics in independent groups and to compare the post-test of each experimental group with that of the controlling group. In addition, ANOVA analytical method was performed to harmonize experimental and controlling group, to compare the effect of 3 kinds of music over running of athletes and non - athletes and to compare the difference of post - test and pre - test of running race in non - athletes and runners. The conclusions show that electronic music with slow and fast rhythm and applying desirable music over runners and non - athletes have not a meaningful effect. Besides, the amount of music effect over speed running of runners in comparison with non - athletes has not meaningful difference.