The aim of this study was to determine the effects of high - intensity interval training (HIT) programme on maximal oxygen uptake (VO2max) and 3000m running performance in moderately trained male runners. VO2max , the running speed associated with VO2max (vVO2max ), the time for which vVO2 max can be maintained (T max ) and 3000m running time (3000mTT) were determined before and following HIT programme performed twice per week for 10 weeks. Following the pre-test, 16 moderately trained male runners (VO2max=51.6±2.7 ml.kg-l.min-1) were divided into training and control groups based on their 3000m running performance (Training group, N =8, 5-8 x 50 - 60 %T max @ V∆50- vVo2max,1:2 work: recovery ratio; control group, GCON, N =8, 55 – 65 min @ 75% vVO2max). In Training group, significant improvement (p < 0.05) following HIT were found in VO 2max (+8,4%), vVO2max (+6%), T max(+35 , 4%), and 3000mTT (-7%). No significant change in any variable was found in GCON. The present study has shown that 3000m running performance, VO2max vVO2 max and T max can be significantly developed using HIT programme in moderately trained runners.