The purpose of the present study was to compare the area and bone mineral content (BMC) between female futsal players and non-athlete females. Fifteen female futsal players (age: 24.3±2.8 years, height: 161.1±4.4 cm, weight: 54.3±7.0 kg) and 15 healthy non-athlete females (age: 23.8±2.0 years, height: 160.9±7.0 cm, weight: 51.5±9.6 kg) participated in this study. All of the subjects were healthy without any history of illnesses, osteoporosis or taking medications or history of fracture. Area and BMC were measured by Dual Energy X- Ray Absorptiometry (DEXA) at the lumbar spines (L2-L4), femoral neck and femoral trochanter of both legs. Independent t-tests was used to analysis the data. The result of this study showed that in futsal players, BMC values of lumbar spines, dominant and non-dominant leg (53607.0±2477.8, 35130.6±2393.3, 36791.2±2177.8 mg) were significantly higher than non-athletes (45877.3±1979.2, 27082.8±2178.8, 26739.2±1848.4 mg), (P<0.05). But differences between area in lumbar spines, dominant and non-dominant leg of participants were not significant (42.4±2.5, 31.2±1.8, 31.2±1.9 cm2 versus 41.5±2.5, 30.9±1.9, 30.4±1.9 cm2), (P>0.05). It seems that futsal lead to increase in BMC but it can't effect on bone area. Therefore, increasing BMD was the most important factor to improve the BMC in futsal players.